eIF4E gene mutations and potyvirus resistance

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or...

Reexamination Certificate

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C800S279000, C800S298000, C800S317000, C800S280000, C435S005000, C435S006120

Reexamination Certificate

active

07919677

ABSTRACT:
The invention concerns a method for obtaining potyvirus resistant plants exhibiting one or several mutations in a preserved region of the eIF4E translation factor, defined by the following general sequence (I):DX1X2X3X4KSX5QX6AWGSSX7RX8X9YTFSX10VEX11FWX12X13YNNIHX14P SKLX15X16GAD wherein: —X1, X2, X3, X4, X6, X7, X8, X9, X10, X12, X13, X15, et X16 represent each a neutral amino acid; X5 and X14 represent a basic amino acid; X11 represents an acid amino acid; D, K, S, Q, A, W, G, R, Y, T, F, V, E, N, I, H, P, and L have their usual one-letter code meaning.

REFERENCES:
patent: 99 43820 (1999-09-01), None
patent: 01 40490 (2001-06-01), None
Ruffel et al. Mol. Gen Genomics (2005) 274:346-353.
Whitman, S. et al. “Selectable viruses and altered susceptibility mutants inArabidopsis thaliana” Proc. Natl. Acad. Sci. USA, vol. 96, pp. 772-777 Jan. 1999.
Leonard, Simon et al. “Complex Formation between Potyvirus VPg and Translation Eukaryotic Initiation Factor 4E Correlates with Virus Infectivity” Journal of Virology, The American Society for Microbiology, US, vol. 74, No. 17, pp. 7730-7737 Sep. 2000.
Revers, F. et al. “New Advances In Understanding the Molecular Biology of Plant/Potyvirus Interactions” Molecular Plant-Microbe Interactions, APS Press, St. Paul, MN, US, vol. 12, No. 5, pp. 367-376 1999.
Lellis, A. et al. “Loss-of-Susceptibility Mutants ofArabidopsis thalianaReveal an Essential Role for eIF(iso)4E during Potyvirus Infection” Current Biology, vol. 12, No. 12, pp. 1046-1051 Jun. 25, 2002.

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