Ehrlichia disulfide bond formation proteins and uses thereof

Details Ehrlichia disulfide bond formation proteins and uses thereof Ehrlichia disulfide bond formation proteins and uses thereof

2008-09-30

2010-11-02

Mondesi, Robert B (Department: 1645)

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Bacterium or component thereof or substance produced by said...

C424S184100, C530S350000, C536S023100, C536S023700, C435S069100, C435S069300, C435S069700

Reexamination Certificate

active

07824692

ABSTRACT:
Novel genes encoding homologous immunoreactive thio-disulfide oxidoreductases, or disulfide bond formation (Dsb) proteins fromEhrlichia chaffeensisandEhrlichia canisare disclosed. While theE. chaffeensisandE. canisDsb proteins are at most only 31% or less homologous to other known Dsb proteins, theEhrlichiaDsbs contain a cysteine active site, Cys-Gly-Tyr-Cys, similar to those in known Dsb proteins. As predicted by 15-amino acid identical N-terminal signal peptides, the proteins are primarily localized in the periplasm ofE. chaffeensisandE. canis, possibly playing a role in antigenicity and pathogenesis. The present invention provides the nucleotide and amino acid sequences and expression vectors for theE. chaffeensisandE. canisdsb genes, antisera directed against the proteins, and kits to determine whether an individual or animal is infected with a given species ofEhrlichia.

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