Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Nonplant protein is expressed from the polynucleotide
Reexamination Certificate
2006-01-12
2011-10-04
Page, Brent T (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Nonplant protein is expressed from the polynucleotide
C800S278000, C800S284000, C800S290000, C435S320100, C435S468000
Reexamination Certificate
active
08030543
ABSTRACT:
The invention describes a highly improved, reproducible and a consistent method of transformation and regeneration that results in obtaining 12-15% transgenic plants. The present invention relates to a method of selecting genetically transformed Sunflower explants based on their ability to utilize Xylose as a sole carbohydrate source. Further disclosed is the nucleic acid sequence of the Xylose Isomerase gene, vector construction for incorporation of the selection marker gene and the process ofAgrobacteriumMediated Transformation of target host plant with the vector comprising the gene encoding the enzyme Xylose isomerase under the functional combination of the heterologous regulatory sequences. Also disclosed is the method of selecting the putative transformants post transformation with the said vector that possess a metabolic advantage of utilizing Xylose as a sole carbon source. Increased efficiency of regeneration, better growth and survival has been observed on subjection to the described method of positive selection. The subject invention alleviates the disadvantages of negative selection methods such as the undesired elimination of the transformed cells and the potential environmental harm caused due to the dispersal of the antibiotic and the herbicide resistant genes.
REFERENCES:
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patent: WO-94/20627 (1994-09-01), None
patent: WO-96/24667 (1996-08-01), None
Haldrup, A. et al: “The xylose isomerase gene from Thermoanaerobacterium thermosulfurogenes allows effective selection of transgenic plant cells using D-xyloe as the selection agent” Plant Molecular Biology, Nijhoff/Junk, The Hague, NL, vol. 37, May 1998, pp. 287-296, XP002129151, ISSN: 0167-4412.
Sankara Rao K et al: “Agrobacterium-Mediated Transformation of Sunflower (Helianthus annuusL.) A Simple Protocol”, Annals of Botany, Academic Press, London, GB, vol. 83, No. 4, 1999, pp. 347-354, XP002953938, ISSN: 0305-7364.
Karur Rajyashri Ramakrishna
Patell Villoo Morawala
Avesthagen Limited
Hamre Schumann Mueller & Larson P.C.
Page Brent T
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