Efficient screening methods for low probability variants

Details Efficient screening methods for low probability variants Efficient screening methods for low probability variants

1982-05-27

1986-07-29

Marantz, Sidney

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 4, 435 29, 435 34, 435291, 435297, 435299, 435810, 436519, C12Q 100, C12M 116, C12M 118

Patent

active

046031057

ABSTRACT:
Rapid screening of a large number of organisms for variants producing particular products is accomplished using membrane plates having a predetermined molecular weight cutoff and which divide a container into two chambers. High density cellular lawns are employed in conjunction with labeled antibodies for the product of interest mixed in soft agar. The cells are subjected to viral lysis and after the plaques have reached maturity, the plates are immersed in buffer. Residual soluble labeled antibody diffuses from the agar, and immunoprecipitate of the product and the antibody may then be detected.

REFERENCES:
patent: 3645687 (1972-02-01), Nerenberg
patent: 4326028 (1982-04-01), Brown
patent: 4421849 (1983-12-01), Breuker
patent: 4446234 (1984-05-01), Russo
D. A. Kaplan et al., Gene, 13(3), 211-220, (Apr. 1981).

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