Efficient prokaryotic expression system using portions of the E.

Details Efficient prokaryotic expression system using portions of the E. Efficient prokaryotic expression system using portions of the E.

1984-12-26

1988-01-26

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

4351723, 435201, 435253, 435320, 435849, 536 27, 935 14, 530350, C12P 2100, C12N 1500, C12N 926, C12N 120, C12N 700, C07H 1512, C07K 1300

Patent

active

047216719

ABSTRACT:
A novel highly effective prokaryotic expression system is exemplified specifically by being used to produce the useful enzyme .beta.-glucuronidase (BG). This system uses a hybrid plasmid comprising BG gene promotor DNA. The level of expression of BG by an E. coli K-12 derivative host is in the 50% of total cellular protein range. The invention expression system also can be used to express other useful proteins, as disclosed herein.

REFERENCES:
Hieber, Biochem. and Biophys. Res. Comm., 104(4):1271-1278, 1982, (Feb.).
Ashwell, G. (1962), Methods in Enzymol., 5:190-208.
Ritzenthaler, P. et al., (1983), Mol. Gen. Genet., 191:263-270.
Novel, M. and Novel, G., (1976), J. Bacteriol., 127:406-432.
Blanco, C. et al., (1982), J. Bacteriol., 149:587-594.
Clarke, L. and Carbon, J., (1976), Cell, 9:91-99.

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