Efficient prokaryotic expression system

Details Efficient prokaryotic expression system Efficient prokaryotic expression system

1988-09-30

1990-08-28

Mays, Thomas D.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

435 697, 435 698, 4351723, 435320, 935 47, 935 48, 935 73, C07K 1300, C12P 2100, C12N 1500

Patent

active

049526820

ABSTRACT:
A novel highly effective prokaryotic expression system is exemplified specifically by being used to produce the useful enzyme .beta.-glucuronidase (BG). This system uses a hybrid plasmid comprising BG gene promoter DNA. The level of expression of BG by an E. coli K-12 derivative host is in the 50% of total cellular protein range. The invention expression system also can be used to express other useful proteins, as disclosed herein.

REFERENCES:
patent: 4356270 (1982-10-01), Itakura
Hieber. Biochem. and Biophys. Res. Comm. 104(4):1271-1278, 1982.
Blanco. J. Bacteriol. 149:587-594, 1982.
Ritzenthaler. Mol. Gen. Genet. 191:263-270, 1983.
Ashwell. Meth. in Enzymol. 5:190-208, 1962.

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