Efficient method to conduct large-scale genome sequencing

Details Efficient method to conduct large-scale genome sequencing Efficient method to conduct large-scale genome sequencing

1995-05-10

1997-09-23

Jones, W. Gary

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 5, 435 912, 536 243, 536 2433, 536 2432, C12Q 160, C12Q 178, C12P 1934, C07H 2104

Patent

active

056703210

ABSTRACT:
An efficient method for sequencing large fragments of DNA is described. A subclone path through the fragment is first identified; the collection of subclones that define this path is then sequenced using transposon-mediated direct sequencing techniques to an extent sufficient to provide the complete sequence of the fragment.

REFERENCES:
Roach et al. Genomics 26: 345-353 1995.
Stratham et al. PNAS 88: 1247-1250 1991.
Michael Strathmann, et al., Proc. Natl. Acad. Sci. USA vol. 88, pp. 1247-1250, Feb. 1991, Transposon-facilitated DNA sequencing.
Ellson Y. Chen, et al., Genomics 17, 651-666 (1983) Ordered Shotgun Sequencing, a Strategy for Integrated Mapping and Sequencing of YAC Clones .

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