Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1996-11-14
2000-10-03
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 5, 435 6, 536 2432, 536 2532, C12Q 170, C12Q 168, C07H 2104
Patent
active
061271152
ABSTRACT:
The present invention provides a method for detecting the presence of a predetermined infectious agent in a human or animal subject comprising obtaining a preselected portion of a whole blood sample containing at least one intact blood cell from the subject and detecting the presence of a nucleic acid from the predetermined infectious agent in the intact blood cell by nucleic acid hybridization. The preselected portion of the blood sample can include any portion which contains at least one intact blood cell, for example a peripheral blood smear or cytospin buffy coat preparation. The intact blood cell can be any blood cell including, but not limited to white blood cells and red blood cells. In one embodiment, the blood cell is a lymphocyte.
REFERENCES:
patent: 5008182 (1991-04-01), Sninsky et al.
patent: 5225326 (1993-07-01), Bresser et al.
patent: 5491073 (1996-02-01), Noteborn et al.
patent: 5538871 (1996-07-01), Nuovo et al.
Anon Detection of transgenic animals without cell culture using fluorescence in situ hybridization Bio Techniquew, vol. 18 (6), p. 952, 954 and 956, 1995.
Ven Den Berg et al. Detection of different developmental stages of malaria parasites by non-radioactive DNA in situ hybridization Histochemical Journal vol. 23, p. 109-115, 1991.
Gunseberg et al. Competitive Assay to Improve the Specificity of Detection of Single-Point Mutation in .alpha..sub.1 -Antitypsin Deficiency, Clinical Chemistry 39(10) p. 2157-2162, (1993).
Allen et al. In situ hybridization for the detection of chicken anemia virus in formalin-fixed paraffin-embedded sections Avian Diseases vol. 37, p. 177-183, 1993.
Zawatzky et al. Identification of individual interferon-producing cells by in situ hybridization, Proc. Natl. Acad. Sci. USA, vol. 82 p. 1136-1140, 1985.
Daniele Chevrier, et al., PCR Product Quantification by Non-radioactive Hybridization Procedures using an Oligonucleotide Covalently Bound to Microwells, 1993 Academic Press Limited, Molecular and Cellular Probes (1993) 7, 187-197.
Jean-Robert Deverre, et al., A Competitive Enzyme Hybridization Assay for Plasma Determination of Phosphodiester and Phosphorothioate Antisense Oligonucleotides, 1997 Oxford University Press, 3584-3589 Nucleic Acids Research, 1997, vol. 25, No. 18.
Yuasa et al., "Isolation and some characteristics of an agent inducing anemia in chicks," Avian Diseases, vol. 23, pp. 366-385 (1979).
Gelderblom et al., "Morphological characterization of chicken anemia agent (CAA)," Archives of Virology, vol. 109, pp. 115-120 (1989).
Todd et al., "Purification and biochemical characterization of chicken anemia agent," Journal of General Virology, vol. 71, pp. 819-823 (1990).
Taniguchi et al., "Haematopathological changes in dead and moribund chicks induced by chicken anemia agent," National Institute of Animal Health Quarterly, Japan, vol. 22, pp. 61-69 (1982).
Taniguchi et al., "Chronological observations on hemato-pathological changes in chicks inoculated with chicken anemia agent," National Institute of Animal Health Quarterly, Japan, vol. 23, pp. 1-12 (1983).
Bulow et al., "Untersuchungen uber den Erreger der infektiosen Anamie bie Huhnerkuken (CAA) in vitro: Vermehrung, Titration, Serumneutralisationstest und indirekter Immunofluoreszenztest," Zbl. of Veterinary Medicine, B, vol. 32, pp. 679-693 (1985).
Yuasa et al., "Aetiological examination of an outbreak of haemorrhagic syndrome in a broiler flock in Japan," Avian Pathology, vol. 16, pp. 521-526 (1987).
Jeurissen et al., "Transient depletion of cortical thymocytes induced by chicken anemia agent," Thymus, vol. 14, pp. 115-123 (1989).
Otaki et al., "Isolation of chicken anemia agent and Marek's disease virus from chickens vaccinated with turkey herpesvirus and lesions induced in chicks by inoculating both agents," Avian Pathology, vol. 16, pp. 291-306 (1987).
Cloud et al., "Immune dysfunction following infection with chicken anemia agent and infectious bursal disease virus I. Kinetic alterations of avian lymphocyte subpopulations," Veterinary Immunology and Immunopathology, vol. 34, pp. 3337-3352 (1992).
Cloud et al, "Immune dysfunction following infection with chicken anemia agent and infectious bursal disease virus II. Alterations of in vitro lymphoproliferation and in vivo immune responses," Veterinary Immunology and Immunopathology, vol. 34, pp. 353-366 (1992).
Bounous et al., "Immunosuppression and intracellular calcium signaling in splenocytes from chicks infected with chicken anemia virus, CL-1 isolate," Avian Diseases, vol. 39, pp. 135-140 (1995).
Yuasa et al., "Effect of infectious bursal disease virus infection on incidence of anemia by chicken anemia agent," Avian Diseases, vol. 24, pp. 202-209 (1980).
Bulow et al., "Folgen der Doppelinfektion von Kuken mit Adenovirus oder Reovirus und dem Erreger der aviaren infektiosen Anamie (CAA)," Journal of Veterinary Medicine, B, vol. 33, pp. 717-726 (1986).
Engstrom, B.E., "Blue wing disease of chickens: Isolation of avaian reovirus and chicken anaemia agent," Avian Pathology, vol. 17, pp. 23-32 (1988).
Rosenberger et al., "The effects of age, route of exposure, and coinfection with infectious bursal disease virus on the pathogenicity and transmissibility of chicken anemia agent (CAA)," Avian Diseases, vol. 33, pp. 753-759 (1989).
Rosenberger et al., "The isolation and characterization of chicken anemia agent (CAA) from broilers in the United States," Avian Diseases, vol. 33, pp. 707-713 (1989).
McNulty et al., "Applications of immunofluorescence in veterinary viral diagnosis," Recent advances in virus diagnosis. (McNulty & McFerran, eds.), The Hague: Martinus Nijhoff, Netherlands, pp. 15-26 (1984).
Noteborn et al., "Detection of chicken anaemia virus by DNA hybridization and polymerase chain reaction," Avian Pathology, vol. 21, pp. 107-118 (1992).
Allan et al., "In situ hybridization for the detection of chicken anemia in formalin-fixed, paraffin-embedded sections," Avian Diseases, vol. 37, pp. 177-182 (1993).
Noteborn et al., "Characterization of cloned chicken anemia virus DNA that contains all elements for the infectious replication cycle," Journal of Virology, vol. 65, pp. 3131-3139 (1991).
Sambrook et al., "Molecular cloning: a laboratory manual (Second Edition)," Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y., 1989.
McGadey, J., "A tetrazolium method for non-specific alkaline phosphatase," Histochimie, vol. 23, pp. 180-184 (1970).
Yuasa et al., "Isolation of chicken anemia agent with MDCC-MSB1 cells from chickens in the field," National Institute of Animal Health Quarterly, Japan, vol. 23, pp. 75-77 (1983).
Mitchell et al., "In situ hybridization: a review of methodologies and applications in the biomedical sciences," Medical Laboratory Sciences, vol. 49, pp. 107-118 (1992).
Martinez et al., "Non-radioactive Localization of Nucleic Acids by Direct In Situ PCR and In Situ RT-PCR in Paraffin-embedded Sections," Journal of Histochemistry and Cytochemistry, vol. 43, No. 0, pp. 0-00, 1995.
Todd et al., "Development of an enzyme-linked immunosorbent assay to detect serum antibody to chicken anemia agent," Avian Diseases, vol. 34, pp. 359-363 (1990).
Noteborn et al., "Chicken anemia virus infection: molecular basis of pathogenicity," Avian Pathology, vol. 24, pp. 11-31 (1995).
McNulty et al., "Economic effects of subclinical chicken anemia agent infection in broiler chickens," Avian Diseases, vol. 35, pp. 263-268 (1991).
Yuasa et al., "Survey of antibody against chicken anaemia agent (CAA) by an indirect immunofluorescent antibody technique in breeder flocks in Japan," Avian Pathology, vol. 14, pp. 521-530 (1985).
Chan, V. T-W, Extraction of nucleic acids from clinical samples and cultured cells. In Diagnostic molecular pathology: a practical approach. (Herrington et al., eds.), vol. 2, pp. 1-23, New York: Oxford University Press, 1992.
Herrington et al., Principles and basic methodology of DNA/RNA detection by in situ hybridization. In Diagnostic molecular pathology: a practical approach (Herrington et al. eds.), vol. 1, pp. 69-102, New York: Oxford University Press, 1992.
Winberg, G., "A rapid method
Goodwin Mark A.
Novak Renata
Ragland William L.
Jones W. Gary
Tung Joyce
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