Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1994-12-19
1997-04-22
Jones, W. Gary
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 911, 435879, 435968, 536 231, 536 243, 536 2432, 536 2532, 935 5, 935 8, 935 76, 935 77, 935 78, C12Q 168, C12P 1934, C07H 2104, C12N 1500
Patent
active
056228252
ABSTRACT:
A novel method for conjugating haptens, ligands or luminescent labels to polynucleotides has been developed. This method involves the formation of a mixed anhydride, followed by reaction of the mixed anhydride intermediate with a nucleophilic group-containing polynucleotide in a DMF/water solvent system. The resulting compound finds usefulness especially in the gene probe area.
REFERENCES:
patent: 4745181 (1988-05-01), Law et al.
Agrawal, S. et al, Efficient Methods for Attaching Non-Radioactive Labels to the 5' Ends of Synthetic Oligodeoxyribonucleotides, Nucleic Acids Res., 14 (1986) 6227.
Chollet, A. et a, Biotin-Labeled Synthetic Oligodeoxyribonucleotides: Chemical Synthesis and Uses as Hybridization Probes, Nucleic Acids Res., 13(1985) 1529.
Clontech Laboratories, Inc., DNA Modification Reagents For Use in Automated DNA Synthesis, 1989.
Schroeder, H. R. et al, Monitoring Specific Protein-Binding Reactions with Chemiluminescence, Methods in Enzymology, 57(1978) 424.
STN, Registry file, RN 82029-66-1, sequence (nucleotide) comparison with probe SYOMPA 480.24.
Law Say-Jong
Lukinsky Hana
Ciba Corning Diagnostics Corp.
Jones W. Gary
Morgenstern Arthur S.
Roesler Judith A.
Sisson Bradley L.
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