Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2011-03-22
2011-03-22
Vogel, Nancy (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S069100, C435S071100
Reexamination Certificate
active
07910330
ABSTRACT:
A method of efficiently expressingPlasmodiumAMA-1 ectodomain or a functional part, derivative, and/or analogue thereof, in a eukaryotic expression system. Preferably, thePlasmodiumAMA-1 ectodomain is Pf AMA-1 ectodomain. This protein may be expressed in yeast, such asPichia pastoris. Efficient expression is possible using a method for producing mRNA encoding thePlasmodiumAMA-1 ectodomain in a yeast cell, comprising providing the yeast cell with a nucleic acid encodingPlasmodiumAMA-1 ectodomain, the nucleic acid being modified to utilize the yeast cell's codon usage. Preferably, at least one putative yeast polyadenylation consensus sequence in the nucleic acid has been modified. More preferably, also at least one site in the protein that is generally glycosylated by eukaryotic expression systems, has been removed.
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Blackman Michael John
Holder Anthony Arthur
Kocken Clemens Hendricus M.
Thomas Alan William
Withers-Martinez Chrislaine
Hibbert Catherine
Stichting Biomedical Primate Research Centre
TraskBritt
Vogel Nancy
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