Effective nuclear reprogramming in mammals using CDK2...

Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal – Via microinjection of a nucleus into an embryo – egg cell – or...

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C800S014000, C800S015000, C800S016000, C800S017000, C800S018000, C435S375000

Reexamination Certificate

active

06906238

ABSTRACT:
The present invention provides methods of producing a cloned non-human mammalian nuclear transfer (NT) embryo and methods for producing a cloned non-human mammal. Embodiments of the methods include introducing donor genetic material into a metaphase I oocyte; introducing donor genetic material into a non-enucleated oocyte; introducing donor genetic material obtained from a donor cell that is at metaphase into an oocyte; introducing donor genetic material into an oocyte, and naturally activating the oocyte or the NT embryo; and introducing donor genetic material obtained from a donor cell that is at late G1 phase into an oocyte.

REFERENCES:
patent: 4994384 (1991-02-01), Prather et al.
patent: 5057420 (1991-10-01), Massey
patent: 5096822 (1992-03-01), Rosenkrans, Jr. et al.
patent: 5160312 (1992-11-01), Voelkel
patent: 5213979 (1993-05-01), First et al.
patent: 5366888 (1994-11-01), Fry et al.
patent: 5453366 (1995-09-01), Sims et al.
patent: 5480772 (1996-01-01), Wangh
patent: 5496720 (1996-03-01), Susko-Parrish et al.
patent: 5651992 (1997-07-01), Wangh
patent: 5773217 (1998-06-01), Wangh
patent: 5945577 (1999-08-01), Stice et al.
patent: 6235969 (2001-05-01), Stice
patent: 0 521 674 (1993-01-01), None
patent: 2 340 943 (2000-02-01), None
patent: WO 90/13627 (1990-11-01), None
patent: WO 95/16770 (1995-06-01), None
patent: WO 96/07732 (1996-03-01), None
patent: WO 97/07668 (1997-03-01), None
patent: WO 97/07669 (1997-03-01), None
patent: WO 98 30683 (1998-01-01), None
patent: WO 98/07841 (1998-02-01), None
patent: WO 98/39416 (1998-09-01), None
Collas et al (1992) Influence of Cell Cycle Stage of the Donor Nucleus on Development of Nuclear Transplant Rabbit Embryos. Biology of Reproduction. vol. 46, pp. 492-500.
Yang et al (1992) Nuclear Totipotency of Cultured Rabbit Morulae to Support Full-Term Development Following Nuclear Transfer. Biology of Reproduction. vol. 47, 636-643.
Meirelles et al (2001) Complete Replacement of the Mitochondrial Genotype in a Bos indicus calf Reconstituted by Nuclear Transfer to a Bos taurus Oocyte. Genetics. May 2001, vol. 158, pp. 351-356.
Fehilly et al (1985) Cytogeentic and Blood Group Studies of Sheep/Goat Chimaeras. J. Reprod. Fert. 1985., vol. 74, pp. 215-221.
Compton et al. Am Improved Method for Routine Preparation of Intact Artificial Chromosome DNA (340-1000 kb) for Transfection into Human Cells. Nucleic Acids Res. 1999, vol. 27, No. 7, pp. 1762-1765.
Chastant-Maillard et al., “Clonage et Reprogrammation du Noyau dans I'embryon Préimplantatoire,”Gynécol Obstét Ferril, (2000), vol. 28, pp. 649-658.
Keefer et al., “Effect of Follicle-Stimulating Hormone and Luteinizing Hormone During Bovine In Vitro Maturation on Development Following In Vitro Fertilization and Nuclear Transfer,”Molecular Reproduction and Development,(1993), vol. 36, pp. 469-474.
Kikyo et al., “Reprogramming Nuclei: Insights From Cloning, Nuclear Transfer and Heterokaryons,”Journal of Cell Science, (2000), vol. 113, pp. 11-20.
Miyoshi et al., “Development of Porcine Embryos Reconstituted with Somatic Cells and Enucleated Metaphase I and II Oocytes Matured in a Protein-free Medium,”BMC Developmental Biology, (2001), vol. 1.
Polejaeva et al., “New Advances in Somatic Cell Nuclear Transfer: Application in Transgenesis,”Theriogenology, (2000), vol. 52, No. 1, pp. 117-126.
Stice et al., “Cloning: New Breakthroughs Leading to Commercial Opportunities,”Theriogenology, (1998), vol. 49, pp. 129-138.
Abeydeera et al., “Glutathione content and embryo development after in vitro fertilisation of pig oocytes matured in the presence of a thiol compound and various concentrations of cysteine,”Zygote. Aug. 1999;7(3):203-10.
Abeydeera et al., “Development and viability of pig oocytes matured in a protein-free medium containing epidermal growth factor,”Theriogenology.Sep. 15, 2000;54(5):787-97.
Alessi et al., “The cyclin-dependent kinase inhibitors olomoucine and roscovitine arrest human fibroblasts in G1 phase by specific inhibition of CDK2 kinase activity,”Exp Cell Res.Nov. 25, 1998;245(1):8-18.
Baguisi et al., “Induced encleation in nuclear transfer procedures to produce cloned animals,”Theriogenology.Jan. 1, 2000; 53(1):209.
Barnes et al., “Influence of recipient oocyte cell cycle stage on DNA synthesis, nuclear envelope breakdown, chromosome constitution, and development in nuclear transplant bovine embryos,”Mol Reprod Dev.Sep. 1993;36(1):33-41.
Betthauser et al., “Production of cloned pigs from in vitro systems,”Nat Biotechnol.Oct. 2000;18(10):1055-9.
Campbell et al., “Sheep cloned by nuclear transfer from a cultured cell line,”Nature. Mar. 7, 1996;380(6569):64-6.
Cheong et al., “Birth of mice after transplantation of early cell-cycle-stage embryonic nuclei into enucleated oocytes,”Biol Reprod.May 1993;48(5):958-63.
Cibelli et al., “Cloned transgenic calves produced from nonquiescent fetal fibroblasts,”Science.May 22, 1998;280(5367):1256-8.
Collas et al., “Nuclear transplantation by microinjection of inner cell mass and granulosa cell nuclei,”Mol Reprod Dev.Jul. 1994;38(3):264-7.
Compton, “New tools for the antimitotic toolbox,”Science.Oct. 29, 1999; 286(5441):913-4.
Curnock et al., “Embryo transfer in pigs: a method for introducing genetic material into primary specific-pathogen-free herds,”Am J Vet Res.Jan. 1976:37(1):97-8.
De Sutter et al., “Parthenogenetic activation of human oocytes by puromycin,”J Assist Reprod Genet.Aug. 1992;9(4):328-37.
De Vos et al., “In-vitro matured metaphase-I oocytes have a lower fertilization rate but similar embryo quality as mature metaphase-II oocytes after intracytoplasmic sperm injection,”Hum Reprod.Jul. 1999;14(7):1859-63.
Du et al., “Development of nuclear transfer embryos using porcine fetal fibroblasts,”Theriogenology, Jan. 1, 1999;51(1):201.
Gandhi et al., “Substrate utilization in porcine embryos cultured in NCSU23 and G1.2/G2.2 sequential culture media,”Mol Reprod Dev.Mar. 2001;58(3):269-75.
Gibbons, John R., “Novel Pig Cloning Procedures for Xenotransplantation,” Grant Abstract, Grant No. 1R43HL065806-01 [online]. National Heart, Lung, and Blood Institute, National Institutes of Health, project dates Sep. 1, 2001-Feb. 28, 2001 [retrieved on Sep. 24, 2000]. Retrieved from the Internet: URL:<http://commons.cit.nih.gov/crisp3/crisp_lib.getdoc?textkey=6211180&p_grant_num=1R43HL065806-01&p_query=&ticket=89400&p_audit_session_id=113185 1&p_keywords=>, 2 pages.
Goto et al., “Birth of cloned calves derived from cultured oviductal epithelial cells of a dairy cow,”Anim Sci J.1999;70(4):243-245.
Graham, “The fusion of cells with one- and two-cell mouse embryos,”Wistar Inst Symp Monogr.1969;9:19-35.
Gulyas, “Ultrastructural observations on rabbit, hamster and mouse eggs following electrical stimulation in vitro,”Am J Anat.Oct. 1976;147(2);203-18.
Hazeleger et al., “State of the art in pig embryo transfer,”Theriogenology. Jan. 1, 1999;51(1):81-90.
Hill et al., “Clinical and pathologic features of cloned transgenic calves and fetuses (13 case studies),”Theriogenology. Jun. 1999;51(8):1451-65.
Inoue et al., “Activation of mitogen-activated protein kinase during meiotic maturation in porcine oocytes,”Zygote.Aug. 1995;3(3):265-71.
Kato et al., “Eight calves cloned from somatic cells of a single adult,”Science. Dec. 11, 1998;282(5396):2095-8.
Kikuchi et al., “Cytoplasmic maturation for activation of pig follicular oocytes cultured and arrested at metaphase I,”J. Reprod Fertil.May 1999;116(1):143-56.
Kubota et al., “Six cloned calves produced from adult fibroblast cells after long-term,”Proc Natl Acad Sci U S A. Feb. 1, 2000;97(3):990-5.
Kühholzer et al., “P

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Effective nuclear reprogramming in mammals using CDK2... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Effective nuclear reprogramming in mammals using CDK2..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Effective nuclear reprogramming in mammals using CDK2... will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3503344

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.