Ectopic pregnancy test

Details Ectopic pregnancy test Ectopic pregnancy test

1991-07-18

1993-08-17

Rosen, Sam

Chemistry: analytical and immunological testing

Pregnancy or ovulation

436518, 436510, 436536, G01N 3353

Patent

active

052368467

ABSTRACT:
A method for determining ectopic pregnancy in pregnant persons comprises obtaining a test sample; and determining the absence of a fetal restricted antigen in the sample. The sample is obtained from the vaginal cavity in the vicinity of the cervical canal or the cervical os. One fetal restricted antigen is fetal fibronectin. In one embodiment of this invention, the sample is contacted with an insoluble support to which anti-(fetal restricted antigen) antibody is adhered, and the fetal restricted antigen binding to the support is determined. Alternatively, a class of substances of which the fetal restricted antigen is a member is captured with a general binding antibody such as an anti-(fibronectin) antibody; an anti-(fetal restricted antigen) antibody such as anti-(fetal fibronectin) antibody is bound to the support; and the absence of binding with fetal restricted antigen is determined. Competition or sandwich assay procedures can be used. Reagents and reagent kits are also included.

REFERENCES:
Hess et al, Obstet. Gynecol., 68:25-28 (1986).
Kuusela et al, Scand. J. Immunol., 12:331-337 (1980).
Ruoslahti et al, Int. J. Cancer, 27:763-767 (1981).

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