Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism
Reexamination Certificate
2011-08-09
2011-08-09
Hobbs, Lisa (Department: 1657)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving viable micro-organism
C435S006150, C435S014000
Reexamination Certificate
active
07993870
ABSTRACT:
The present invention is a method of extracting infectious pathogens from a volume of blood including the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The fibrin lysis reagent is preferably composed of plasminogen and streptokinase frozen in coincident relation until the fibrin lysis reagent is needed whereby streptokinase enzymatically reacts with plasminogen to form plasmin upon thawing. The plasminogen is suspended in an aqueous salt solution prior to freezing including NaCl and Na3PO4.
REFERENCES:
patent: 4693972 (1987-09-01), Mansour et al.
patent: WO 02/090539 (2002-11-01), None
Faux, S. P. et al. “Calcium chelator Quin-2 prevents crocidolite-induced DNA strand breakage in human white blood cells”Mutation Research, 1994, pp. 209-215, vol. 311.
Titball, R. W. “Bacterial Phospholipases C”Microbiological Reviews, Jun. 1993, pp. 347-366, vol. 57, No. 2.
Polymerase Chain Reaction for Rapid Diagnosis of Candidemia;Bull Acad Mil Med Science; Sep. 1998, vol. 22, No. 3.
Al-Soud et al.; Purification and Characterization of PCR-inhibitory Components in Blood Cells;Journal of Clinical Microbiology; p. 485-493; Feb. 2000.
Archibald et al.; Comparison of BACTEC MYCO/F LYTIC and WAMPOLE Isolator 10 (Lysis-Centrifugation) . . . ;Journal of Clinical Micro.; p. 2994-2997; Aug. 2000.
Bernhardt et al.; Detection of Bacteria in Blood by Centrifugation and Filtration;Journal of Clinical Microbiology; p. 422-425; Mar. 1991.
Brannon et al.; Clinical Comparison of Lysis-Centrifugation and Radiometric Resin Systems for Blood Culture;Journal of Clinical Microbiology; p. 886-887; Nov. 1986.
Cao et al.; The Purification and Characterization of a Phospholipase A in Hamster Heart . . . ;Journal of Biological Chemistry; vol. 262, No. 35; p. 16927-16935; Dec. 15, 1987.
Cassels et al.; The Interaction of Streptokinase-Plasminogen Activator Complex, Issue-Type Plasminogen Activator, Urokinase and Their Acylated . . . ; Journal of Biochem; p. 395-400; 1987.
Castellino; Biochemistry of Human Plasminogen; Seminars in Thrombosis & Hemostasis; vol. 10, No. 1; 1984.
Chen et al.; 1-Cys Peroxiredoxin, a Bifunctional Enzyme with Glutathione Peroxidase and Phospholipase A2 Activities;Journal Biological Chemistry; vol. 275; p. 28421-28427; Sep. 2000.
Siersema et al.; Blood Culture Bottles are Superior to Lysis-Centrifugation Tubes for Bacteriological Diagnosis . . . ;Journal of Clinical Microbiology; p. 667-669; Mar. 1992.
Hamilton et al.; Effect of Delay in Processing on Lysis-Centrifugation Blood Culture Results from Marrow Transplant Patients;Journal of Clinical Microbiology; p. 1588-1593; Jul. 1989.
Hoffman et al.; Bone Marrow Aspirate Culture Superior to Streptokinase Clot Culture and 8 ML 1:10 Blood-To-Broth . . . ;American Society of Tropical Medicine&Hygiene; p. 836-839; 1986.
Morata et al.; Diagnostic Yield of a PCR Assay in Focal Complications of Brucellosis;Journal of Clinical Microbiology; p. 3743-3746; Oct. 2001.
Li et al.; Effects of Volume and Periodicity on Blood Cultures;Journal of Clinical Microbiology; p. 2829-2831; Nov. 1994.
Klevezas et al.; Single-Step PCR for Detection ofBrucellaspp, from Blood and Mild of infected Animals;Journal of Clinical Microbiology; p. 3087-3090; Dec. 1995.
Ortuno et al.; Rapid Diagnosis of Human Brucellosis by Peripheral-Blood PCR Assay;Journal of Clinical Microbiology; p. 2927-2930; Nov. 1997.
Queipo-Ortuno et al.; Rapid Diagnosis of Human Brucellosis by Peripheral-Blood CRR Assay;Journal of Clinical Microbiology; P9. 2927-2930; Nov. 1997.
Gamboa et al.; Detection and Identification of Mycobacteria by Amplification of RNA and DNA in Pretreated Blood and Bone . . . ;Journal of Clinical Microbiology; p. 2124-2128; Aug. 1997.
Morata et al.; Posttreatment Follow-Up of Brucellosis by PCR Assay;Journal of Clinical Microbiology; p. 4163-4166; Dec. 1999.
Miles et al.; Binding and Activation of Plasminogen on the Platelet Surface;Journal of Biological Chemistry; vol. 260, No. 7; p. 4303-4311; Apr. 1985.
Gaffney et al.; Plasma Fibrinogen and its Fragments During Streptokinase Treatment;British Journal of Haematology; 1974.
Malin et al.; Effect of Tetrahydropyrimidine Derivatives on Protein-Nucleic Acids Interaction;Journal of Biological Chemistry; vol. 274; p. 6920-6929; Mar. 12, 1999.
Malin et al.; Induction of Synthesis of Tetrahydropyrimidine Derivatives inSteptomycesStrains and Their Effect onEscherichia coli.; Journal of Bacteriology; p. 385-395; Jan. 1996.
Phospholipase A2 from Bee Venom;Methods in Enzymology, vol. 71; 1981.
Nguyen et al.; Thrombolysis Using Liposomal-Encapsulated Streptokinase: An In Vitro Study; vol. 192;P.S.E.B.M.; 1989.
Birnboim, H.C.; Rapid Extraction of High Molecular Weight RNA from Cultured Cells & Granulocytes for Northern Analysis;Nucleic Acids Research; vol. 16, No. 4; 1988.
Fisher et al.; Lysosomal-type PLA2 & Turnover of Alveolar DPPC; Lung Cellular & Molecular Physiology; vol. 280; p. 74-754; Apr. 2001.
Shipolini et al.; Phospholipase A from Bee Venom;Journal Biological Chemistry; p. 459-468; 1971.
Menashe et al.; Hydrolysis of Dipalmitoylphosphatidylcholine Small Unilamellar Vesicles by Porcine Pancreatic . . . ;Journal of Biological Chemistry; vol. 261, No. 12; p. 5328-5333; 1986.
Molloy et al.; Proteomic Analysis of theEscherichia coliOuter Membrane;Journal of Biological Chemistry; p. 2871-2881; 2000.
Sponer et al.; Electronic Properties, Hydrogen Bonding, Stacking, and Cation Binding of DNA and RNA Bases; Institute of Biophysics; Aug. 2001.
Gonzalez et al.; Mechanism of Action of Polymeric Aurintricarboxylic Acid, a Potent Inhibitor of Protein-Nucleic Acid Interactions;American Chemical Society; 1980.
Oberbaumer et al.; Detection of RNA on Northern Blots by Negative Staining with Aurinlricarboxylic Acid;Journal Biochemistry; p. 77-79; 1990.
Givens et al.; Inhibition of RNA-directed DNA Polymerase by Aurintricarboxylic Acid; Nucleic Acids Research; vol. 3, No. 2; Feb. 1976.
Hallick et al.; Use of Aurintricarboxylic Acid as an Inhibitor of Nucleases During Nucleic Acid Isolation; Nucleic Acids Research; vol. 4, No. 9; Sep. 1977.
Guo et al.; Aurin Tricarboxylic Acid Directly Activates Platelets; Thrombosis Research; p. 77-88; 1993.
Gonzalez et al.; Fractionation and Structural Elucidation of the Active Components of Aurintricarboxylic Acid, A Potent Inhibitor of Protein Nucleic Acid . . . ;Biomedical Press; 1979.
Skidmore et al.; Characterization and Use of the Potent Ribonuclease Inhibitor Aurintricarboxylic Acid for the Isolation of RNA..Journal of Biological Chemistry; p. 73-80; 1989.
Tsutsui et al.; Fractionation of Aurintricarboxylic Acid & Effects of its Components on Nuclear Swelling & Nucleic Acid Synthesis;Biomedical Press; p. 14-23; 1978.
Nakane et al; Differential Inhibition of Various Deoxyribonucleic Acid Polymerases by Evans Blue & Aurintricarboxylic Acid;Journal of Biological Chemistry; p. 91-96; 1988.
Rozalski et al.; Effects of Fibrinogen Receptor Antagonist GR144053F & Aurintricarboxylic Acid on Platelet Activation and Degranulation;Biochemical Pharmacology; p. 1399-1408; 2001.
Browne et al.; Binding Studies of Cationic Thymidyl Deoxyribonucleic Guanidine to RNA Homopolynucleotides; Jul. 1995.
Huang et al.; Electric Manipulation of Bioparticles and Macromolecules on Microfabricated Electrodes;Analytical Chemistry; vol. 73, No. 7; Apr. 1, 2001.
Cheng et al.; Isolation of Cultured Cervical Carcinoma Cells Mixed with Peripheral Blood Cells on a Bioelectronic Chip;Analytical Chemistry; vol. 70, No. 11; Jun. 1, 1998.
Li et al.; Transport, Manipulation, and Reaction of Biological Cells On-Chip Using Electrokinetic Effects;Analytical Chemistry; vol. 69, No. 8; Apr. 15, 1997.
Yang et al.; Granted, Stacked Microlaboratory for Biological Agent Detection with DNA & Immunoassays;BioSensors&BioElectronics; p. 605-618; 2002.
Edman et al; Electric Field Directed Nucleic Acid Hybridization on Microchips;Nucleic Acids Research; vol. 25, No. 24; 1997.
Ewalt et al; De
Amuso Philip
Cannons Andrew
Ewert Matt
Hobbs Lisa
Saliwanchik Lloyd & Eisenschenk
University of South Florida
LandOfFree
Early detection of pathogens in blood does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Early detection of pathogens in blood, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Early detection of pathogens in blood will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2776545