Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing alpha or beta amino acid or substituted amino acid...
Reexamination Certificate
2004-11-23
2009-12-29
Fronda, Christian L (Department: 1652)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing alpha or beta amino acid or substituted amino acid...
C435S183000, C435S252300, C536S023200
Reexamination Certificate
active
07638312
ABSTRACT:
The present invention relates to a Tryptophan-producingE. colimutant strain CJ285 (KCCM-10534) containing single or multi mutant genes related with Tryptophan biosynthesis and production method of Tryptophan using the same. More particularly, DNA base sequences and amino acid sequences aroF, aroG, trpR, and tyrR originated from tryptophan producingE. colimutant strain CJ285 (KCCM-10534) and related with Tryptophan biosynthesis, are disclosed, andE. coliCJ285 containing at least one of the mutant genes is cultivated directly in a glucose-containing fermentation medium, whereby L-tryptophan can be accumulated in the culture medium.
REFERENCES:
Storbakk et al. J Mol Biol. Mar. 15, 1996;256(5):889-96.
Kim Byoung-Hoon
Kim Seong-Jun
Lim Ho-Soo
Lim Sang-jo
Park Young-Hoon
Buchanan Ingersoll & Rooney P.C.
CJ Cheiledang Corp.
Fronda Christian L
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