E. coli msDNA synthesizing system, products and uses

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical


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435 9141, 536 232, 536 252, C12N 1552, C12N 1566, C12P 1934




A multicopy single-stranded DNA (msDNA) synthesizing system in E. coli is disclosed. The use of the msDNA system to synthesize cDNA in vivo is disclosed. Construction of synthetic msDNA is also disclosed. Also processes for gene amplification and for producing a stable RNA are disclosed.

patent: 5405775 (1995-04-01), Inouye et al.
patent: 5436141 (1995-07-01), Miyata et al.
Dhundale et al., J. Bact., vol. 170, Dec. 1988, pp. 5620-5624.
Dhundale et al., Cell, vol. 51, 1987, pp. 1105-1112.


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