E. coli-derived upstream regulatory sequence operable in yeast

Details E. coli-derived upstream regulatory sequence operable in yeast E. coli-derived upstream regulatory sequence operable in yeast

1991-08-28

1995-06-13

Schwartz, Richard A.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

435 691, 43525421, 935 36, 935 37, C12N 1511

Patent

active

054244110

ABSTRACT:
A hybrid upstream regulatory sequence including an upstream regulatory sequence of E. coli origin and yeast upstream regulatory sequence, which hybrid upstream regulatory sequence can function in a yeast cell; a hybrid promoter including the regulatory sequence of E. coli origin and a TATA region of a yeast promoter, which hybrid promoter can also function in a yeast cell; a hybrid promoter including the upstream regulatory sequence of E. coli origin and a yeast promoter, which hybrid promoter can function in a yeast cell; and a plasmid useful for a test of an upstream regulatory sequence, including a TATA region of a yeast promoter, a structural gene containing a translation start codon, and a yeast origin of replication.

REFERENCES:
patent: 4833080 (1989-05-01), Brent et al.
patent: 5089398 (1992-02-01), Rosenberg et al.
Kwak et al., "A Method to Isolate DNA Sequences that are Promoter Active in Escherichia coli and in yeast," Biochem. Biophy. Res. Comm. 149(3):846-851 (Dec. 1987).
Goodley et al., "The Selection of Promoters for the Expression of Heterologous Genes in the Yeast Saccharomyces cerevisiae," Mol. Gen. Genet. 204:505-511 (1986).
Sidhu et al., Yeast, vol. 6, No. 3, 1990, pp. 221-229.
Ciaramella et al., Nucleic Acids Research, vol. 16, No. 18, 1988, pp. 8846-8868.

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