E. coli and Streptomyces host cells that contain MatBC...

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

Reexamination Certificate

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C435S252350, C435S252330

Reexamination Certificate

active

06939691

ABSTRACT:
The use of enzymes that catalyze the production of starter and extender units for polyketides inE. coliandStreptomycesis described; these enzymes include malonyl CoA decarboxylase (MatA), malonyl CoA synthetase (MatB), and a malonate transporter (MatC) as well as proprionyl CoA carboxylase (pcc). The matBC gene fromStreptomyces coelicolor, the matABC genes fromRhizobium trifoli, and the pccB and accA2 fromStreptomyces coelicolorare useful in specific embodiments of the claimed invention. These enzymes may be used to enhance the yield of polyketides that are natively produced or polyketides that are rationally designed. By using these techniques, the synthesis of a complete polyketide has been achieved inE. coliin the presence of a phosphopantetheinyl transferase, such as sfp fromBacillus subtilis. This achievement permits a host organism with desirable characteristics to be used in the production of such polyketides and to assess the results of gene shuffling.

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