Dynamic continuous flow enzyme reactor

Details Dynamic continuous flow enzyme reactor Dynamic continuous flow enzyme reactor

1988-02-08

1989-09-12

Jones, Larry

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

435291, C12M 140

Patent

active

048659843

ABSTRACT:
A dynamic continuous flow enzyme reactor and method for carrying out and analyzing phospholipid-dependent enzyme reactions are provided. The enzyme reactor is a tubular member preferably a capillary tube, coated on its inner surface with a planar phospholipid bilayer membrane, optionally and preferably containing an enzyme or enzyme cofactor. The reactor is connectable at one end to a means for delivering fluid flow reagents to the reactor and is connectable at a second end to a means for analyzing an effluent exiting from the reactor. Preferably the enzyme cofactor in the phospholipid membrane is purified tissue factor and the fluid reagents are inactive blood clotting factors which become activated upon interaction with the phospholipid membrane through a tissue factor-mediated reaction.

REFERENCES:
patent: 3785772 (1974-01-01), Coggeshall
patent: 4361484 (1982-11-01), Larson et al.
patent: 4452682 (1984-06-01), Takata et al.
patent: 4604182 (1986-08-01), Seago
Lottenberg et al., "Assay of Coagulation Proteases", Methods in Enzymology, 0: 341-361.
Bach et al., "Factor VII Binding to Tissue Factor in Reconsituted Phospholipid Vesicles: Induction of Cooperativity by Phosphatidylserine", Biochemistry, 25: 4007-4020 (1986).
Brian et al., "Allogenic Stimulation of Cytotoxic T Cells by Supported Planar Membranes", Proc. Natl. Acad. Sci. USA, 81: 6159-6163 (Oct. 1984).
Nemerson et al., "Progress in Hemostasis and Thrombosis", Spaet, T. H. edit., Grune & Stratton, New York, vol. 6, pp. 237-261 (1982).
Carson, Prog. Clin. Pathol., 9: 1-4 (1984).
Nemerson, Blood, vol. 71, No. 1, pp. 1-8 (1988).
Zur et al., "The Dual Role of Factor VII in Blood Coagulation", J. Biol. Chem., 257: 5623-5631 (1982).
Ragni et al., "Factor VII Deficiency", Amer. J. Hematol., 10: 79-88 (1981).

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