Dyeing agent having at least two dyes for staining a biological

Organic compounds -- part of the class 532-570 series – Organic compounds – Azo

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8639, 436 17, 436 63, 436174, 436805, 435 405, 435 4051, G01N 3300, G01N 33493, G01N 3348, G01N 3349

Patent

active

055854699

ABSTRACT:
Dyeing agents which are excellent in visual recognition of discernible or tangible components in a sample cause no coagulation of proteins, sugars or glycoproteins dissolved in the sample. An apparatus for image analysis of flow type stain particles uses the dyeing agents, and particles of discernible components suspended in a flowing sample can be detected so that images of the particles can be efficiently photographed and the discernible components can thus be analyzed by image processing of the thus-obtained image.

REFERENCES:
patent: 3822095 (1974-07-01), Hirschfeld
patent: 3961039 (1976-08-01), Sternheimer
Venkataraman, "The Chemistry of Synthetic Dyes", vol. VII, pp. 278-305 (1974).
D. Lapen, "A Standardized Differential Stain for Hematology", Cytometry, vol. 2, No. 5, Mar. 1982, pp. 309-315.
A. W. Krause et al, "Fluorescent Erythrosin B is Preferable to Trypan Blue as a Vital Exclusion Dye for Manmmalian Cells in Monolayer Culture", The Journal of Histochemistry and Cytochemistry, vol. 32, No. 10, Oct., 1984, pp. 1084-1090.

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