Dye-providing composition, diagnostic test kit and their use in

Details Dye-providing composition, diagnostic test kit and their use in Dye-providing composition, diagnostic test kit and their use in

1987-12-18

1991-06-18

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 75, 435 79, 435 792, 435 794, 435 28, 435188, 435180, 435810, 436501, 436518, 436536, 436538, 436543, 436547, 436808, 436818, 436824, 526246, 5263031, 5263171, 528422, 528425, 548346, G01N 3353, G01N 33536, C12Q 128, C12N 996

Patent

active

050249355

ABSTRACT:
A dye-providing composition comprises a water-soluble or -dispersible polymer, such as a vinylpyrrolidone polymer, and an imidazole leuco dye capable of providing a dye in the presence of hydrogen peroxide and a peroxidative substance. The weight ratio of polymer to leuco dye is from about 10,000:1 to about 100:1. The dye-providing composition can be included with a peroxidase substrate in a diagnostic test kit. A method for the determination of a ligand can be carried out using a peroxidase labeled-receptor for the ligand and the dye-providing composition described above. The method is particularly useful for the determination of human chorionic gonadotropin (hCG).

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