Dye-labeled antibody conjugate and preparation method thereof

Details Dye-labeled antibody conjugate and preparation method thereof Dye-labeled antibody conjugate and preparation method thereof

1997-04-02

1999-07-13

Smith, Lynette F.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is organic

436800, 435 71, 5303871, 524900, 424 149, G01N 33549, C07K 1600

Patent

active

059226188

ABSTRACT:
The invention relates to a dye-labeled antibody conjugate comprising an antibody conjugate and a cyanine dye. The dye-labeled antibody conjugate is prepared by polymerizing an antibody such as mouse IgG in phosphate buffer using a polyfunctional reagent such as dithiobis(sulfosuccinimidylpropionate), and stirring the antibody and a cyanine dye represented by Formula I . The dye-labeled antibody conjugate is more sensitive to antigens than conventional dye-labeled antibodies because it has many reactive sites to antigens. ##STR1## wherein X represents a halogen, M represents a hydrogen or alkali metal, and n represents an integer of 1 to 4

REFERENCES:
patent: 4069352 (1978-01-01), Parsons
Mar. 1998, Communication from European Patent Office and attached Search Report.
Mujumdar, et al., "Cyanine Dye Labeling Reagents: Sulfoindocyanine Succinimidyl Esters", Bioconjugate Chemistry, vol. 4, No. 2, Mar./Apr. 1993, pp. 105-111.
Staros (1982) Membrane impermeant cleavable cross-linkers, Biophys. J., vol. 37, pp. 21-22.

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