Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1997-11-12
1999-11-09
Benzion, Gary
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
536 231, 435 691, 435468, 435469, 435410, 435419, 435418, 435412, 800278, 800284, 800295, 8003201, C12N 1529, C12N 504, C12N 1582, A01H 500
Patent
active
059817283
ABSTRACT:
The maize gene dull1 (du1) of the present invention is a determinant of the structure of endosperm starch. Mutations of du1 affect the activity of at least two enzymes involved in starch biosynthesis, namely the starch synthase, SSII, and the starch branching enzyme, SBEIIa. Du1 codes for a predicted 1674 residue protein, and is expressed with a unique temporal pattern in endosperm but is undetectable in leaf or root. The size of the Du1 product and its expression pattern match precisely the known characteristics of maize SSII. The Du1 product contains two different repeated regions in its unique amino terminus, one of which is identical to a conserved segment of the starch debranching enzymes. The cDNA provided for in the present invention encodes SSII, and mutations within this gene affect multiple aspects of starch biogenesis by disrupting an enzyme complex containing starch synthase(s), starch branching enzyme(s), and possibly starch debranching enzyme(s).
REFERENCES:
Matzke et al. Plant Physiol. 1995. 107:679-685.
Napoli et al. The Plant Cell. 1989. vol. 2: 278-289.
James Martha G.
Myers Alan M.
Adler Benjamin Aaron
Benzion Gary
Iowa State University Research Foundation
Zaghmout Ousama M-Faiz
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