Dual tag binding assay

Details Dual tag binding assay Dual tag binding assay

1996-09-06

1999-06-08

Horlick, Kenneth R.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 6, 435 5, 435 4, 435 912, 435 717, 435 79, 436 56, 436 94, 436501, 536 243, 536 2433, 536 231, C12Q 168

Patent

active

059104104

ABSTRACT:
A method for detecting the presence of a target nucleic acid species having first, second and third target sequences. In the present invention, a solution to be tested is brought into contact with a solid support having a probe nucleic acid species attached thereto, the probe nucleic acid sequence comprising a sequence that is complementary to the first target sequence. A dye solution is then brought into contact with the solid support. The dye solution includes a first dye attachment nucleic acid sequence coupled to a first dye and a second dye attachment nucleic acid sequence coupled to a second dye. The first dye attachment nucleic acid sequence is complementary to the second target sequence and the second dye attachment nucleic acid sequence is complementary to the third target sequence. The ratio of concentrations of the first and second dye attachment nucleic acid sequences in the dye solution is different from the ratio of the first and second dye attachment sequences specifically bound by the target nucleic acid species. By measuring the ratio of the first and second dyes, non-specific binding of the dye attachment sequences can be detected. In one embodiment of the invention, the target nucleic acid sequence comprises a fourth target sequence, and the dye solution includes a third dye attachment nucleic acid sequence complementary to the fourth target sequence and coupled to a third dye.

REFERENCES:
patent: 4563419 (1986-01-01), Ranki et al.
patent: 5521065 (1996-05-01), Whiteley et al.
patent: 5565322 (1996-10-01), Heller
patent: 5589585 (1996-12-01), Mabilat et al.

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