Dual-state nutritional medium for the transport and maintenance

Details Dual-state nutritional medium for the transport and maintenance Dual-state nutritional medium for the transport and maintenance

1994-02-10

1997-01-14

Marx, Irene

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435 11, 4352402, 4352404, 4352403, 43524054, 435260, 4353052, 4353053, 4353054, 4353071, C12N 502, C12M 118, C12M 116, A01N 102

Patent

active

055938809

ABSTRACT:
A nutritional medium for enabling the growth, maintenance and transport of cells in a container of virtually any size or shape, including a substantially flat, multi-well plastic plate. The nutritional medium is capable of assuming a substantially liquid state above a first temperature and a substantially solid state below a second temperature. Both the first and second temperatures are generally compatible with the survival of the cells in the container. The cells are covered with the medium while the medium is in a substantially liquid state, and the temperature of the medium is then reduced to a temperature at which the medium assumes a substantially solid state. The medium is maintained in a substantially solid state while the cells are transported. Upon arrival at the end user, the temperature of the medium is increased to a temperature at which the medium assumes a substantially liquid state. Following a change to fresh maintenance medium, the cells in the container are ready for immediate use.

REFERENCES:
patent: 4148689 (1979-04-01), Hino et al.
patent: 5004805 (1991-04-01), Gohda et al.
patent: 5045454 (1991-09-01), Bertheussen
patent: 5157110 (1992-10-01), Kotwal et al.
patent: 5314814 (1994-05-01), Harder et al.
ATCC Catalogue of Cell Lines & Hybridomas. 6th Edition. 1988 pp. i-xii.

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