Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases
Reexamination Certificate
2006-02-14
2006-02-14
Saidha, Tekchand (Department: 1652)
Drug, bio-affecting and body treating compositions
Enzyme or coenzyme containing
Hydrolases
C435S196000, C435S198000, C435S252300, C435S320100, C536S023200, C530S350000
Reexamination Certificate
active
06998120
ABSTRACT:
This invention relates to dual roles of the pepper esterase protein as a biocontrol agent that can elicit defense reactions in plant cells and as direct fungal inhibitor that can block fungal penetration into plant cells. The possible enzymatic actions of the PepEST protein were elucidated in both fungus and plant to gain insight into the molecular mechanisms involved in the fungal resistance. Exogenously treated PepEST protein on the unripe pepper fruits decomposed the cuticles of the fruits and released glycerol and 9-octadecenamide. The treatment resulted in the massive generation of hydrogen peroxide in the unripe pepper fruit and simultaneously elicited the expression of defense-related genes in both absence and presence of the fungus.
REFERENCES:
patent: 6252049 (2001-06-01), Oh et al.
Kim et al. Molecular Plant-Microbe Interaction, vol. 14(1): 80-85 (Jan. 2001).
Baudouin et al.; Eur. J. Biochem. 248, 700-706, 1997:Functional expression of a tobacco gene related to the serine hydrolase family.
Carvalho, et al.; EJB Electronic Journal of Biotechnology ISSN:0717-345, vol. 1, No. 3, 1998:Cutinase structure, function and biocatalytic applications.
Warm et al.; PHYTOCHEMISTRY, vol. 21, No. 4, pp. 827-831, 1982:Quantification of Hydrogen Peroxide in Plant Extracts by the Chemiluminescence Reaction with Luminol.
Falk et al.; Proc. Natl. Acad. Sci. USA, vol. 96, pp. 3292-3297, Mar. 1999, Plant Biology:ESD1, an essential component of R gene-mediated disease resistance in Arabidopsis has homology to eukaryotic lipases.
Fauth, et al..; Plant Physiol. (1998) 117:1373-1380:Cutin Monomers and Surface Wax constituents Elicit H2O2in Conditioned Cucumber Hypocotyl Segments and Enhance the Activity of Other H2O2Elicitors.
Jirage, et al.: PNAS, vol. 96, No. 23, 13583-13588, 1999:Arabidopsis thaliana PAD4 encodes a lipase-like gene that is important for salicyclic acid signaling.
Oh, et al.; J. Phytopathology 146, 301-303, 1998:A Microscopic Characterization of the Invection of Green and Red Pepper Fruits by an Isolate of Colletotrichum gloeosporioides.
Schweitzer et al.; The Plant Journal (1996) 10(2), 331-341:Perception of free cutin monomers by plant cells.
Kim Young Soon
Ko Moon Kyung
Oh Boung-Jun
Seo Hyo Hyoun
Song Pill-Soon
Korea Kumho Petrochemical Co. Ltd.
Saidha Tekchand
LandOfFree
Dual roles of pepper esterase as a biocontrol agent does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Dual roles of pepper esterase as a biocontrol agent, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dual roles of pepper esterase as a biocontrol agent will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3635305