Dual roles of pepper esterase as a biocontrol agent

Drug – bio-affecting and body treating compositions – Enzyme or coenzyme containing – Hydrolases

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C435S196000, C435S198000, C435S252300, C435S320100, C536S023200, C530S350000

Reexamination Certificate

active

06998120

ABSTRACT:
This invention relates to dual roles of the pepper esterase protein as a biocontrol agent that can elicit defense reactions in plant cells and as direct fungal inhibitor that can block fungal penetration into plant cells. The possible enzymatic actions of the PepEST protein were elucidated in both fungus and plant to gain insight into the molecular mechanisms involved in the fungal resistance. Exogenously treated PepEST protein on the unripe pepper fruits decomposed the cuticles of the fruits and released glycerol and 9-octadecenamide. The treatment resulted in the massive generation of hydrogen peroxide in the unripe pepper fruit and simultaneously elicited the expression of defense-related genes in both absence and presence of the fungus.

REFERENCES:
patent: 6252049 (2001-06-01), Oh et al.
Kim et al. Molecular Plant-Microbe Interaction, vol. 14(1): 80-85 (Jan. 2001).
Baudouin et al.; Eur. J. Biochem. 248, 700-706, 1997:Functional expression of a tobacco gene related to the serine hydrolase family.
Carvalho, et al.; EJB Electronic Journal of Biotechnology ISSN:0717-345, vol. 1, No. 3, 1998:Cutinase structure, function and biocatalytic applications.
Warm et al.; PHYTOCHEMISTRY, vol. 21, No. 4, pp. 827-831, 1982:Quantification of Hydrogen Peroxide in Plant Extracts by the Chemiluminescence Reaction with Luminol.
Falk et al.; Proc. Natl. Acad. Sci. USA, vol. 96, pp. 3292-3297, Mar. 1999, Plant Biology:ESD1, an essential component of R gene-mediated disease resistance in Arabidopsis has homology to eukaryotic lipases.
Fauth, et al..; Plant Physiol. (1998) 117:1373-1380:Cutin Monomers and Surface Wax constituents Elicit H2O2in Conditioned Cucumber Hypocotyl Segments and Enhance the Activity of Other H2O2Elicitors.
Jirage, et al.: PNAS, vol. 96, No. 23, 13583-13588, 1999:Arabidopsis thaliana PAD4 encodes a lipase-like gene that is important for salicyclic acid signaling.
Oh, et al.; J. Phytopathology 146, 301-303, 1998:A Microscopic Characterization of the Invection of Green and Red Pepper Fruits by an Isolate of Colletotrichum gloeosporioides.
Schweitzer et al.; The Plant Journal (1996) 10(2), 331-341:Perception of free cutin monomers by plant cells.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Dual roles of pepper esterase as a biocontrol agent does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Dual roles of pepper esterase as a biocontrol agent, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dual roles of pepper esterase as a biocontrol agent will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3635305

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.