Dual reporter system and methods of use therefor

Details Dual reporter system and methods of use therefor Dual reporter system and methods of use therefor

1998-10-22

2000-05-16

Schwartzman, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4353201, 435366, 435371, C12Q 168, C12N 510, C12N 1585

Patent

active

060635789

ABSTRACT:
Plasmid reporter systems for assaying transcription and replication are provided. The plasmids comprise dual reporters for use in the independent evaluation of transcription and replication. Such plasmids may be used for example, for determining the role of endogenous factors in transcription and replication and for evaluating the effect of exogenous modulators on such processes. Within certain embodiments, the plasmids comprise (a) a first reporter gene operably linked to a promoter and a binding site for human papillomavirus E1 and/or E2 protein; (b) a second reporter gene that is linked to a promoter that is not substantially modulated by E1 and/or E2 and (c) an origin of replication bound by human papillomavirus E1 protein.

REFERENCES:
patent: 5620849 (1997-04-01), Botchan et al.
patent: 5928888 (1999-07-01), Whitney
patent: 5965393 (1999-10-01), Hasnain et al.
Breiding et al., "Functional Interaction of a Novel Cellular Protein with the Papillomavirus E2 Transactivation Domain," Molecular And Cellular Biology 17(12): 7208-7219, 1997.
Ferguson and Botchan, "Genetic Analysis of the Activation Domain of Bovine Papillomavirus Protein E2: Its Role in Transcription and Replication," Journal Of Virology 70(7): 4193-4199, 1996.
Frattini and Lamins, "The Role of the E1 and E2 Proteins in the Replication of Human Papillomavirus Type 31b," Virology 204: 799-804, 1994.
Huang et al., "Potential Replication of Recombinant Baculoviruses in Nontarget Insect Species: Reporter Gene Products as Indicators of Infection," Journal Of Invertebrate Pathology 69: 234-245, 1997.
Lambert, "Papillomavirus DNA Replication," Journal Of Virology 65(7): 3417-3420, 1991.
Monini et al., "Activation of Eukaryotic Transcriptional Prometers by the Bovine Papillomavirus E1-Replication Factor," Intervirology 36: 245-252, 1993.
Powell et al., "Inhibition of cellular activation of retroviral replication by CD8.sup.+ T cells derived from non-human primates," Clin. Exp. Immunol. 91: 473-481, 1993.
Ustav and Stenlund, "Transient replication of BPV-1 requires two viral polypeptides encoded by the E1 and E2 open reading frames," The EMBO Journal 10(2): 449-457, 1991.

Affiliated with

Also associated with

Rating

Dual reporter system and methods of use therefor does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Dual reporter system and methods of use therefor, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dual reporter system and methods of use therefor will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-257461