Dual particle immunoassay method and kit

Details Dual particle immunoassay method and kit Dual particle immunoassay method and kit

1997-09-30

2000-08-01

Chin, Christopher L.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is particulate and the particles are of...

435 71, 435 72, 435 792, 435 795, 436518, 436524, 436533, 436536, 436538, 436540, 436808, 436810, 436824, G01N 33533

Patent

active

060965630

ABSTRACT:
A dual particle immunoassay method and kit for detecting analyte in a sample in which the sample to be analyzed, a binding molecule specific for the analyte, and a particle coated with the analyte to be detected or coated with a second binding molecule are reacted and applied to a porous membrane. The competitive immunoassay utilizes an analyte-coated particle, whereas the sandwich immunoassay employs a second binding molecule-coated particle. All of the reagents except for the coated particle are able to pass through the porous membrane. Detectable particles coated with a binding substance that binds to the binding molecule, such as protein A protein G, second antibody reactive to the binding molecule, or a small synthetic affinity ligand, are reacted with coated particles retained on the membrane surface. The detectable particles will pass through the membrane if not complexed with the coated particle. In the competitive immunoassay, detectable particles bind to binding molecules that complex with the analyte-coated particles in the absence of analyte and are detected. In the sandwich immunoassay, detectable particles bind to binding molecules that are attached to the coated particle in the presence of analyte and are detected.

REFERENCES:
patent: Re34405 (1993-10-01), Gould et al.
patent: 4115535 (1978-09-01), Giaever
patent: 4169138 (1979-09-01), Jonsson
patent: 4184849 (1980-01-01), Cambiaso et al.
patent: 4456691 (1984-06-01), Stark
patent: 4469787 (1984-09-01), Woods et al.
patent: 4859612 (1989-08-01), Cole et al.
patent: 4952520 (1990-08-01), Okusa et al.
patent: 5145790 (1992-09-01), Mattingly et al.
patent: 5236826 (1993-08-01), Marshall
patent: 5358851 (1994-10-01), Peck
patent: 5565366 (1996-10-01), Akers, Jr.
"D TECH Field Test Kits" brochure, EM Science & Strategic Diagnostics (Apr. 1995).
"D TECH environmental test kits" brochure, EM Science & Strategic Diagnostics (Jan. 1995).
Eck, D.L., et al., "Trinitrotoluene and Other Nitroaromatic Compounds," Immunochemical Methods for Environmental Analysis, American Chemical Society, pp. 79-94 (1990).
Painless Particles.TM., vol, 6(4), Bangs Laboratories, Inc., Carmel (Dec. 1993).
Albro et al., "A Radioimmunoassay for Chlorinated Dibenzo-p-Doxins," Toxicol. Appl. Pharmacol., vol. 50, pp. 137-146 (1979).
Luster et al., "Radioimmunoassay for Quantitation of 2,3,7,8-Tetrachlorodibenzofuran," Anal. Chem., vol. 52, pp. 1497-1500 (1980).
Vanderlaan et al., "Environmental Monitoring by Immunoassay," Enzyme Sci. Technol., vol. 22, 247-253 (1988).
Vanderlaan et al., "Immunochemical Techniques in Trace Residue Analysis," Immunoassays for Trace Chemical Analysis, Vanderlaan et al., eds., American Chemical Society, Washington, D.C., pp. 2-13 (1990).

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