Dual-luciferase reporter system

Details Dual-luciferase reporter system Dual-luciferase reporter system

1999-03-31

2000-11-07

McKelvey, Terry

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 8, 4353201, C12Q 168, C12Q 166, C12N 1579

Patent

active

061435023

ABSTRACT:
Plasmids and methods of use for assaying translational recoding are disclosed. The plasmids contain a constitutively expressed renilla (Renilla reniformis; sea pansy) luciferase gene, a polylinker for insertion of a selected DNA segment, and an out-of-frame firefly luciferase gene. Recoding is determined by monitoring luminescence of the firefly luciferase normalized to the luminescence of the renilla luciferase.

REFERENCES:
patent: 5744320 (1998-04-01), Sherf et al.
Stahl, Guillaume et al. "Versatile vectors to study recoding: conservation of rules between yeast and mammalian cells" Nucleic Acids Research vol. 23 No. 9 pp. 1557-1560, 1995.
Sambrook, J. et al "Molecular Cloning: A Laboratory Manual" 2d ed. Cold Spring Harbor Laboratory Press, Cold Spring Harobor NY Section 1.7-1.8, 1989.

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