Dual-fluorescence cell viability assay using ethidium homodimer

Details Dual-fluorescence cell viability assay using ethidium homodimer Dual-fluorescence cell viability assay using ethidium homodimer

1991-10-28

1994-05-24

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving viable micro-organism

435 6, 435 34, 435968, 436800, C12Q 102, C12Q 168, C12Q 104

Patent

active

053148053

ABSTRACT:
This invention relates to a method for simultaneously detecting live and dead cells using two fluorogenic reagents: calcein AM and ethidium homodimer. Live cells are distinguished by an intense uniform green fluorescence generated by the enzymatic hydrolysis of calcein AM: ##STR1## Dead or damaged cells are distinguished by a bright red fluorescence resulting from nucleic acids stained with ethidium homodimer: ##STR2## The assay is useful to determine cell viability and to monitor cytotoxicity agents or events.

REFERENCES:
patent: 5001051 (1991-03-01), Miller
Haugland, Handbook of Fluorescent Probes and Research Chemicals, Sets 24 and 28 (1989-1991).
I. C. MacCoubrey, P. L. Moore, R. P. Haugland, Quantitative Fluorescence Measurements of Cell Viability (Cytotoxicity) with a Multi-Well Plate Scanner, J Cell Biol 111, 58A (Abstract 303).
P. L. Moore, I. C. MacCoubrey, R. P. Haugland, A Rapid, ph Insensitive, Two Color Fluorescence Viability (Cytotoxicity) Assay, J Cell Biol 111, 58A (Abstract 304).

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