Optics: measuring and testing – By particle light scattering – With photocell detection
Reexamination Certificate
2006-07-25
2009-12-08
Punnoose, Roy (Department: 2886)
Optics: measuring and testing
By particle light scattering
With photocell detection
C356S340000, C356S343000
Reexamination Certificate
active
07630076
ABSTRACT:
Apparatus, systems and methods for measurement of B22values of proteins in aqueous solutions in flow-mode utilize a dual-detector cell are provided. Simultaneous measurement of protein concentration and scattered light intensity is facilitated as the protein elutes from a size-exclusion column. Each data point on the resulting chromatograms is converted to Rayleigh's ratio, Rθ, and to concentration c, respectively. The B22value is calculated from the slope of the Debye plot (Kc/Rθversus c) generated from a range of the concentrations obtained from these chromatograms for a single protein injection. Measurements may be analyzed using modeling data derived from a predetermined modeling equation to quantify self-association of molecules. The apparatus and method provide a reliable means for determining B22values for such proteins as lysozyme, chymotrypsinogen, and chymotrypsin in various solution conditions.
REFERENCES:
patent: 4573796 (1986-03-01), Martin et al.
patent: 4616927 (1986-10-01), Philips et al.
patent: 4693602 (1987-09-01), Wyatt et al.
patent: 4952055 (1990-08-01), Wyatt
patent: 5250186 (1993-10-01), Dollinger
patent: 5269937 (1993-12-01), Dollinger
patent: 5305071 (1994-04-01), Wyatt et al.
patent: 5305073 (1994-04-01), Ford
patent: 5530540 (1996-06-01), Wyatt et al.
patent: 5676830 (1997-10-01), Janik et al.
patent: 5734464 (1998-03-01), Gibbs
patent: 5936714 (1999-08-01), Gibbs
patent: 6315955 (2001-11-01), Klein
patent: 6404493 (2002-06-01), Altendoft
patent: 6411383 (2002-06-01), Wyatt et al.
patent: 6426405 (2002-07-01), Jeng
patent: 6651009 (2003-11-01), Trainoff et al.
patent: 7440101 (2008-10-01), Auer et al.
patent: 2004/0067167 (2004-04-01), Zhang et al.
patent: 2005/0105077 (2005-05-01), Padmanabhan et al.
patent: 2005/0255600 (2005-11-01), Padmanabhan et al.
patent: 2007/0206203 (2007-09-01), Trainer
Wen, et al., Size-Exclusion Chromatography With On-Line Light-Scattering, Absorbance, And Refractive Index Detectors For Studying Proteins And Their Interactions.Anal. Biochem. 240:155-166, 1996.
Bloustine, et al., Measurements Of Protein-Protein Interactions By Size-Exclusion Chromatography,Biophys. J. 85:2619-2623, 2003.
Wyatt, Light Scattering And The Absolute Characterization Of Macromolecules.Anal. Chim. Acta. 272:1-40, 1993.
Wyatt, Mean Square Radius Of Molecules And Secondary Instrumental Broadening.J. Chromatogr. A. 648:27-32, 1993.
Bajaj, et al., Determination Of Second Virial Coefficient Of Proteins Using A Dual-Detector Cell For Simultaneous Measurement Of Scattered Light Intensity And Concentration In SEC-HPLC, Biophysical Journal, vol. 87, Dec. 2004, pp. 4048-4055.
Jackson, et al., Characterization Of Biopolymers Using A Multi-Angle Light Scattering Detector With Size-Exclusion Chromatography.J. Applied Polym. Sci. 43:99-114, 1989.
Netopilik, Combined Effect Of Interdetector Volume And Peak Spreading In Size Exclusion Chromatography With Dual Detection.Polymers. 38:127-130, 1997.
Netopilik, Problems Connected With Band-Broadening In Size-Exclusion Chromatography With Dual Detection.J. Biochem. Biophys. Meth. 56:79-93, 2003.
Wyatt, et al., The Interdetector Volume In Modern Light Scattering And High Performance Size-Exclusion Chromatography.LC-GC. 11:862-872, 1993.
Zammit, et al., Factors Influencing Detector Matching In Multidetector SEC: Solvent And Concentration Effects.Polymers. 39:5789-5798, 1998.
Harminder Bajaj
Kalonia Devendra S.
Vikas Sharma K.
McCarter & English LLP
Punnoose Roy
University of Connecticut
LandOfFree
Dual-detector systems and methods having utility in... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Dual-detector systems and methods having utility in..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dual-detector systems and methods having utility in... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4144598