DS11 (KCTC 0231BP), novel Bacillus sp. strain and novel...

Details DS11 (KCTC 0231BP), novel Bacillus sp. strain and novel... DS11 (KCTC 0231BP), novel Bacillus sp. strain and novel...

1998-12-16

2001-07-03

Marx, Irene (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S196000

Reexamination Certificate

active

06255098

ABSTRACT:

BACKGROUND OF THE INVENTION
1. Field of the Invention
This invention relates to a novel phytase produced from novel strain Bacillus sp. DS11 (KCTC 0231BP) and more precisely, to a novel strain Bacillus sp. DS11 and phytase enzyme enhancing the phosphate bioavailability present in grains supplied to monogastric animals.
2. Description of the Prior Art
Phytase is an enzyme to degrade phytic acid into phosphate and phosphate inositol. 50 to 70% of phosphate in grain used as livestock feeds exists in form of phytic acid but phytase is not present in monogastric animals such as hens and hogs, resulting in low phosphate availibility.
Therefore undigested phytic acid(phytictaine) released to a water source became one of the serious environment contamination sources causing to eutrophication in small lakes or tides. With above consideration, because monogastric animals can't utilize phytic acid in their intestine phytic acid chelates to water due to chelation with a trace amount of minerals, amino acids and vitamins which are very important to the metabolism of livestock. These formed water-insoluble, undigestable chelate complexes released to feces change the environmental ecosystem to induce a serious environmental pollution.
In view of these situations, the application of phytase into the livestock feeds will reduce the supply of inorganic phosphate due to increase of phosphate bioavailibility in livestock, leading to economic benefits, and improving the availibility of phosphate, and other bioactive substances, leading to reduction of the environmental contamination.
By these reasons, the utilization of phytase in livestock is very important. A law regulating the amount of phosphate in animal waste was established in 1996 in Korea and in European countries it is already mandatory to add phytase in the feeds of animals.
The addition of phytase in the feeds may greatly improve the productivity of livestock by enhancing the availability of some bioactive substances (phosphate, calcium and zinc etc.) which, otherwise chelate with phytictaine and lose their activity.
As the result, the use of feeds containing phytase in livestock can enhance the availibility of feeds and reduce the environmental contamination caused by phosphate.
For the aforementioned benefits, intensive studies about phytase including the effects of phytase on animals Young et al., Addition of microbial phytase to diet of young pigs,
J Anim Sci
71(8)2147-50 (1993); Lei et al., Calcium level affects the efficacy of supplemental microbial phytase in corn-soybean meal diets of weaning pigs,
J Anim Sci
72(1)139-43 (1994); and Mroz et al., Apparent digestibility and retention of nutrients bound to phytate complexes as influenced by microbial phytase and feeding regimen in pigs,
J Anim Sci
27(1):126-32 (1994); Z. Mroz et al., 1994) have been performed mainly in Europe Ullah., A. H., The complete primary structure elucidation of
asperigillus ficuum
(niger), pH 2.5-optimum acid phosphatase by Edman degradation,
Biochem Biophy Res Commun
203(1):182-89 (1994); Ehrlich, et al., Identification and cloning of a second phytase gene (phyB) from
Aspergillus niger
(ficuum); and Piddington et aL, The Cloning and sequencing of the genes encoding phytase (phy) and pH 2.5-optimum acid phosphatase (aph) from
Asperigillus niger
var. awamori,
Gene
133(1):55-62 (1993).However, since phytase can cleave only a limited number of phosphates and it mostly produced by molds which have long growing period, it is uneconomical in terms of mass production. In addition, it is difficult to use the phytic acid as an additive for monogastric animals since it is unsuitable to their physiological mechanism.
SUMMARY OF THE INVENTION
Therefore, the inventor et al. have identified a novel phytase-producing microorganism among hundred kinds of molds, Actinomycetes, bacteria, etc., obtained from soils and barns throughout the country, in an effort to produce phytase having excellent enzymatic potency and shorten the production period compared with the conventional phytase. Because of the high enzymatic potency of this novel microorganism and its physiological suitability to use for monogastric animals and short period of kproduction compared to those of conventional enzymes the inventor et al. have judged that this enzyme has novelty and they have completed this invention.
The object of this invention is to provide novel strain Bacillus sp. DS11 (KCTC 0231BP) and phytase enzyme, which is suitable to use for monogastric animals with excellent properties and more shortened production period.


REFERENCES:
patent: 6-38745 (1994-05-01), None
patent: WO 94/19 471 A1 (1994-01-01), None
Shimizu, M., Biosci., Biotechnol., Biochem. (1992), 56(8), 1266-9.*
Powar et al., J. Bacteriol., vol. 151, p. 1102-1108, 1982.*
Ehrlich et al., Indentification and cloning of a second phytase gene (phyB) fromAspergillus niger(ficuum), Biochem Biophy Res Commun 195:53-7 (1993).
Greiner, et al., Purification and Characterization of Two Phytases fromEscherichia coli, Arch Biochem Biophys 303:107(1993).
Gibson, D.M., Some modification to the media for rapid automated detection of salmonellas by conductance measurement, J Appl Bacteriol 63(4):299-304 (1987).
Lei et al., Calcium level affects the efficacy of supplemental microbial phytase in corn-soybean meal diets of weanling pigs, J. Anim Sci 72(1):139-43 (1994).
Mroz et al., Apparent digestibility and retention of nutrients bound to phytate complexes as influenced by microbial phytase and feeding regimen in pigs, J Anim Sci 72(1):126-32 (1994).
Piddington et al., The Cloning and sequencing of the genes encoding phytase (phy) and pH 2.5-optimun acid phosphatase (aph) fromAspergillus nigervar.awamori, Gene 133(1):55-62 (1993).
Sneath, P., Endospore-forming Gram-Positive Rods and Cocci, Bergey's Manual of Systematic Bacteriology 2:1104-1139 (1984).
Ullah., A.H., The complete primary structure elucidation ofAsperigillus ficuum(niger), pH 6.0, optimum acid phosphatase by Edman degradation, Biochem Biophy Res Commun 203(1):182-9 (1994).
Ullah, A.H., Production, Rapid Purification and Catalytic Characterization of Extracellular Phytase fromAspergillus ficuum, Prep Biochem 18:443-58 (1988).
Ullah, A.H.,Aspergillus ficuumPhytase: Partial Primary Structure, Substrate Selectivity, and Kinetic Characterization, Prep Biochem 18:459-72 (1988).
Ullah, A.H., Immobilization ofAspergillus ficuumPhytase: Product Characterization of the Bioreactor, Prep Biochem 18:483-89 (1988).
Ullah, A.H.,Aspergillus ficuumExtracellular PH 6.0 Optimum Acid Phosphatase: Purification, N-Terminal Amino Acid Sequence, and Biochemical Haracterization, Prep Biochem 18:37-65 (1988).
Young et al., Addition of microbial phytase to diets of young pigs, J Anim Sci 71(8):2147-50 (1993).

Affiliated with

Also associated with

Rating

DS11 (KCTC 0231BP), novel Bacillus sp. strain and novel... does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with DS11 (KCTC 0231BP), novel Bacillus sp. strain and novel..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and DS11 (KCTC 0231BP), novel Bacillus sp. strain and novel... will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2551258