Dry preparation for reticulocyte staining

Details Dry preparation for reticulocyte staining Dry preparation for reticulocyte staining

1978-01-05

1980-03-18

Serwin, R. E.

Drug, bio-affecting and body treating compositions

Radionuclide or intended radionuclide containing; adjuvant...

Molecular bilayer structure

252408, 422 57, 427 4, G01N 3316, G01N 3122, G01N 2160

Patent

active

041939808

ABSTRACT:
A dry stain for reticulocyte staining comprising a mixture of new methylene blue N dye, potassium acetate, and an anti-blood-clotting agent. The stain is prepared in a concentrated aqueous solution, filtered, dispensed volumetrically into tubes, and dried. The correct quantity of dye per tube is determined spectrophotometrically, and the proper ratio of dye to potassium acetate is determined by pH measurements. For staining reticulocytes, whole blood is added to the tube and mixed with the dry stain. Smears are then prepared on slides in a conventional manner for microscopic examination.

REFERENCES:
patent: 4070495 (1978-01-01), Berger et al.
Brecher, "New Methylene Blue as a Reticulocyte Stain,"Amer. Jour. of Clin. Path., vol. 19, pp. 895-896 (1949).
Robertson, "The Effects of Experimental Plethora on Blood Production," Jour. of Experimental Medicine, vol. 26 pp. 221-237 (1917).
Cunningham, "A Method for the Permanent Staining of Reticulated Red Cells," Archives of Inter. Med., vol. 26, pp. 405-409 (1920).
Vander et al., "Reticulocyte Counts by Means of Fluorescence Microscopy," J. Lab. and Clin. Med. 62, p. 132 (1963).
Marshall et al., "Purified Azure B as a Reticulocyte Stain," J. Clin. Path, 29, pp. 1060-1063 (1976).
Orten, "The Properties and Significance of the Reticulocyte," Yale J. Biol. & Med. 6, pp. 519-539 (1934).

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