Dry enzyme formulations containing D-amino acid oxidase

Details Dry enzyme formulations containing D-amino acid oxidase Dry enzyme formulations containing D-amino acid oxidase

1984-10-17

1989-02-21

Warren, Charles F.

Chemistry: molecular biology and microbiology

Carrier-bound or immobilized enzyme or microbial cell;...

Enzyme or microbial cell is immobilized on or in an organic...

435 25, 435188, 435189, C12N 1108, C12N 996, C12Q 126

Patent

active

048064785

ABSTRACT:
A stable formulation of a non-cellulosic fabric having D-amino acid oxidase coated and dried thereon is shown. The fabric may be woven, nonwoven, or knit and may be made from polyolefin, nylon, polyester, or polyurethane fabrics. Polyolefin fabrics may be pretreated with a surfactant. Also shown are formulations containing some or all of the reagents used to detect beta lactam antibiotics. Kits having webs coated with analytically effective amounts of D-amino acid oxidase, peroxidase, flavin adenine, dinucleotide, a peroxide sensitive dye and a D,D-carboxypeptidase substrate containing a carboxyterminal D-alanine are shown. D,D-carboxypeptidase R39 enzyme may be provided separately in either aqueous form or dried on a separate piece of fabric. Under appropriate conditions the D,D-carboxypeptidase may also be dried on the fabric.

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Frere, J. et al, "Enzymatic Method for Rapid and Sensitive Determination of Beta-Lactum Antibiotics' Antimicrobial Agents and Chemotherapy", Oct. 1980, vol. 18, No. 4, pp. 506-510.
Carrea, G. et al, Biochim. Biophys. Acta, vol. 745, 1983, pp. 181-188.

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