Dry chemistry cascade immunoassay and affinity assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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435 5, 435 6, 435 72, 435 74, 435 77, 435 792, 435 793, 435 794, 435 13, 4352885, 4352887, 435810, 436526, 436527, 436 46, 436805, 436806, 436807, 436808, 436809, 436810, 422 57, 422 58, 422 61, 422 73, G01N 3353

Patent

active

056771339

ABSTRACT:
A method is described for performing an affinity assay comprising contacting a sample to be assayed for the presence of an analyte with a dry reagent containing the analyte (hapten, antigen, antibody, receptor, or complementary polynucleotide) bound to a reaction cascade initiator, an antibody or other binding pair partner reactive with said analyte, and magnetic particles, to form an assay mixture in a reaction chamber, incubating the assay mixture, applying an oscillating or moving static magnetic field to the assay mixture, activating the reaction cascade initiator to initiate a reaction cascade, monitoring the response of the magnetic particles to the oscillating or moving static magnetic field to provide a time varying signal, and determining the analyte concentration of the sample by analysis of the time varying signal, as well as a kit for performing the assay and a diagnostic system for performing the assay.

REFERENCES:
patent: 4849340 (1989-07-01), Oberhardt
patent: 5110727 (1992-05-01), Oberhardt
patent: 5350676 (1994-09-01), Oberhardt et al.
patent: 5601991 (1997-02-01), Oberhardt

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