Dry analytical element for acetaminophen assay

Details Dry analytical element for acetaminophen assay Dry analytical element for acetaminophen assay

1992-07-15

2000-01-18

Lee, Howard C.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 4, 435 18, 435 25, 435 28, 435966, 435969, 435970, 436815, C12Q 137, C12Q 126, C12Q 128, C12Q 100

Patent

active

06015683&

ABSTRACT:
A spectrophotometric assay for the detection of acetaminophen in aqueous fluids can be carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; a second enzyme, ascorbic acid oxidase, to oxidize the p-aminophenol so that it couples to a water soluble coupling agent to form a dye that is read at 670 nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.

REFERENCES:
patent: 3992158 (1976-11-01), Przybylowicz et al.
patent: 4258001 (1981-03-01), Pierce et al.
patent: 4292272 (1981-09-01), Kitajima et al.
patent: 4357363 (1982-11-01), Pierce et al.
patent: 4414327 (1983-11-01), Hammond et al.
patent: 4424150 (1984-01-01), Khanna
patent: 4430433 (1984-02-01), Hammond et al.
patent: 4430436 (1984-02-01), Koyama et al.
patent: 4504413 (1985-03-01), Khanna
patent: 4605754 (1986-08-01), Khanna
patent: 4675290 (1987-06-01), Matsumoto et al.
patent: 4845030 (1989-07-01), Batz et al.
patent: 4948727 (1990-08-01), Cass et al.
patent: 4966855 (1990-10-01), Deneke et al.
patent: 4999288 (1991-03-01), deCastro et al.
patent: 5030571 (1991-07-01), Best et al.

Affiliated with

Also associated with

Rating

Dry analytical element for acetaminophen assay does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Dry analytical element for acetaminophen assay, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dry analytical element for acetaminophen assay will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-562241