Dry analytical element containing self-developing substrate for

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Calorimeter

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422 57, 422 58, 435 4, 435 21, 436170, C12Q 100, G01N 2177, G01N 3122, 435, 435, 422, 422, 422, 422, 422, 422, 422, 436

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active

050770110

ABSTRACT:
A dry analytical element for use in the measurement of activity of enzyme in an aqueous liquid, such as, body fluid, having one or more water-retaining layers. The enzyme has an optimum pH in a in pH range within which the dissociation of p-nitrophenol is insufficient for color formation. The layers of the element comprise a self-developing substrate having p-nitrophenol group attached to the molecular structure through an ether or ester linkage and a cationic polymer, said self-developing substrate and said cationic polymer being incorporated separately in different water-retaining carriers which are arranged to allow the layers to have liquid contact with each other.

REFERENCES:
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patent: 4153668 (1979-05-01), Hiu et al.
patent: 4274832 (1981-06-01), Wu et al.
patent: 4557901 (1985-12-01), Koyama et al.
patent: 4576793 (1986-03-01), Koyama et al.
patent: 4671937 (1987-06-01), Katsuyama et al.

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