Dry analytical element and method for the detection of an aminop

Details Dry analytical element and method for the detection of an aminop Dry analytical element and method for the detection of an aminop

1992-06-01

1994-09-06

Wityshyn, Michael G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

435 16, 435 24, 435 25, 435805, 422 56, 422 57, 436169, 436170, C12Q 100, C12Q 137, G01N 2100

Patent

active

053447530

ABSTRACT:
A dry analytical element has been prepared for the assay of leucine aminopeptidase at a pH of 6.5 to 11. The element zone contains an aromatic substrate for the enzyme. This substrate provides an aromatic reactant which has a primary amino group on the aromatic ring in the ortho or para position to an electron donor group. The aromatic reactant is oxidized with an oxidizing compound (such as an oxidase) and the oxidized compound reacts with a ballasted color-forming coupler to provide a dye.

REFERENCES:
patent: 4390343 (1983-06-01), Walter
patent: 4588836 (1986-05-01), Matsumoto
patent: 4681841 (1987-07-01), Matsumoto
patent: 4732736 (1988-03-01), Robayashi
patent: 4776904 (1988-10-01), Charlton

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