Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process...
Patent
1995-07-07
1998-06-30
Naff, David M.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
204403, 4351731, 435174, 435182, 4352831, 4352891, 435382, C12P 100, C12N 1300, C12N 1104, C12N 500
Patent
active
057732388
ABSTRACT:
A reaction chamber is constructed of a reactant-containing aqueous solution, which may be in droplet form, coated with a fluoropolymer powder such as Polytetrafluoroethylene (PTFE), preferably having a particle size of less than 500 microns. After a reaction, the droplet is destroyed by adding a substance such as a detergent or organic solvent, and the fluoropolymer powder is removed by centrifuging and filtering. Using a micropipette, size of the droplet chamber is increased or decreased by removing or adding liquid, or liquid is transferred from one droplet chamber to another. Charcoal, metal powder or silica powder can be inserted inside or on the surface of the droplet. A droplet chamber containing a first reactant such as an enzyme-bound bead is combined with a second droplet chamber containing a second reactant to react the first and second reactants. A dialysis chamber contains a filtration membrane in contact with the droplet chamber. Placing the droplet chamber between two electrodes and producing current flow between the electrodes provides an electrochemical reaction. A temperature-sensitive reaction such as the Polymerase Chain Reaction (PCR) is performed by heating or cooling the droplet chamber. The droplet reaction chamber is advantageous for microvolumes of sample since container walls are not present on which the sample can disperse, and the chamber does not require significant energy for heating or cooling. In tissue culture, coating a solution containing tissue cells with the fluoropolymer powder reduces sticking of the cells to surfaces of a container.
REFERENCES:
patent: 5401634 (1995-03-01), Milbrath
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