Chemical apparatus and process disinfecting – deodorizing – preser – Physical type apparatus – Crystallizer
Patent
1990-12-18
1992-01-07
Chaudhuri, Olik
Chemical apparatus and process disinfecting, deodorizing, preser
Physical type apparatus
Crystallizer
156600, 156DIG62, 156DIG93, C30B 702
Patent
active
050789759
ABSTRACT:
This invention relates to a crystal growth apparatus (10) generally used for growing protein crystals wherein a vapor diffusion method is used for growing the crystals. In this apparatus, a precipitating solution and a solution containing dissolved crystalline material are stored in separate vials (12, 14), each having a resilient diaphragm (28) across one end and an opening (24) with a puncturable septum (26) thereacross at an opposite end. The vials are placed in receptacles (30) having a manifold (41) with a manifold diaphragm (42) in contact with the vial diaphragm at one end of the receptacle and a hollow needle (36) for puncturing the septum at the other end of the manifold. The needles of each vial communicate with a ball mixer (40) that mixes the precipitate and protein solutions and directs the mixed solution to a drop support (64) disposed in a crystal growth chamber (16), the drop support being a tube with an inner bevelled surface (66) that provides more support for the drop (68) than the tubes of the prior art. A sealable storage region (70) intermediate the drop support and mixer provides storage of the drop (68) and the grown crystals.
REFERENCES:
patent: 3013311 (1961-12-01), Meissner
patent: 4517048 (1985-05-01), Shlichta
patent: 4886646 (1989-12-01), Carter et al.
patent: 4909933 (1990-03-01), Carter et al.
patent: 4917707 (1990-04-01), Charmonte et al.
patent: 4919899 (1990-04-01), Hermann et al.
"Microdialysis Crystallization Chamber"; Sieker; Journal of Crystal Growth 90 (1988); pp. 349-357.
Pusey Marc L.
Rhodes Percy H.
Snyder Robert S.
Broad, Jr. Robert L.
Chaudhuri Olik
Garrett Felisa
Seemann Jerry L.
The United States of America as represented by the Administrator
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