Chemical apparatus and process disinfecting – deodorizing – preser – Control element responsive to a sensed operating condition
Reexamination Certificate
1999-07-27
2001-08-28
Ludlow, Jan (Department: 1743)
Chemical apparatus and process disinfecting, deodorizing, preser
Control element responsive to a sensed operating condition
C073S864010, C436S054000, C436S180000
Reexamination Certificate
active
06280689
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates to pipets for dispensing and aspirating a fluid medium. More particularly, the present invention relates to a pipet having an improved tip or dispensing portion which is constructed to reduce the tendency for dripping.
2. Description of Related Art
In laboratory and industrial settings it is well known to use a pipet to extract a certain volume of a fluid medium from one container and to transport and dispense some or all of the extracted volume into another container. Depending upon the nature of each given application, the volume of fluid medium to be extracted and dispensed must be measured accurately. Most pipets employ an open-ended tapered tip through which the fluid medium is drawn and extracted. During such operations, it is critical that the pipet not drip so that accurate volumes are dispensed. Moreover, in certain industrial or scientific manipulations where precise volume deliveries are critical, such as for example, in molecular biology applications including DNA sequencing, in situ hybridization, and enzyme digestion and in certain tissue culture applications, dripless pipet tips are essential.
Typically pipets are generally hollow tubular members which are used by applying suction at an open upper end or mouthpiece in order to extract or aspirate a quantity of fluid medium into the hollow tube. A pressure differential maintained by closing the mouthpiece opening retains the fluid within the pipet allowing transport of the fluid medium to another container. Selective opening of the mouthpiece allows a quantity of the fluid medium contained in the pipet to be dispensed. A certain degree of accuracy in the amount of fluid dispensed is provided by the tapered end portions by reducing the amount of fluid lost due to dripping.
Plastic pipets have largely replaced glass pipets for many uses. Commercially available plastic pipets, however, have a serious draw-back. Plastic pipets typically have a molded or a drawn dispensing tip in the shape of a hollow cone with an open, narrow end. Such tips tend to drip or leak, especially in larger pipets, in which the opening of the cone typically has a greater taper angle to allow for speedy dispensing of larger volumes. Dripping and leaking can be reduced by decreasing the size of the opening or narrowing the taper angle. However this would restrict liquid flow therethrough to an unacceptable level.
Given the nature of pipets and the need for accuracy in the volume of medium which is dispensed, it is common to employ different sized pipets for different volume applications. Large volume pipets, those having a capacity in excess of 50 ml are, quite naturally, of larger size than small volume pipets, those having a capacity under 50 ml.
In many large volume pipets, the size of the dispensing tip is such that it is difficult to control the dispensing of fluid from the pipet. Thus, even upon creation of the pressure differential by closing the mouthpiece, fluid medium has a tendency to drip from the mouthpiece resulting in lack of control in the accuracy of dispensing.
Accordingly, it is desirable to provide a pipet having a dripless tip for use in accurately dispensing specified volumes of a fluid medium which is easy to manufacture and to use.
SUMMARY OF THE INVENTION
The present invention is a pipet tip that can accurately aspirate and dispense a specified volume of fluid medium. Most preferably, the pipet tip includes a structure within the open end of the tip for restricting flow of liquid medium therethrough.
The present invention provides a dripless pipet tip wherein the surface area of the tip is increased by imprinting a cross-shaped structure which increases the effective surface tension of the fluid medium contained therein.
Most preferably, the pipet tip includes an elongate hollow tip body having an open distal end defined by a perimetrical wall. The tip is capable of allowing a fluid medium to pass therethrough. The tip includes a wall structure that spans the open distal end of the tip body. Furthermore, the wall structure extends between at least two spaced apart locations on the perimetrical wall. This wall structure contacts the liquid medium that passes through the open distal end of the tip to effect a reduction in dripping.
The pipet tip further includes a generally circular perimetrical wall that includes an elongated wall member that is diametrically across from the perimetrical wall. The wall structure may further include first and second diametrically extending intersecting wall members. Moreover, the first and second wall members may intersect at a right angle forming a cross-shaped structure. In this embodiment, the elongate hollow tip body is generally frustoconically shaped. In addition, the wall structure may further be physically or chemically etched along its surface. These etched surfaces serve to further increase surface contact between the wall structure and the fluid medium.
Alternatively, the pipet tip includes the cross shaped structure the entire length of the pipet tip. Therefore, more effectively reducing dripping out of the pipet tip.
REFERENCES:
patent: 4147752 (1979-04-01), Suovaniemi et al.
patent: 4923798 (1990-05-01), LeMoine et al.
patent: 5260030 (1993-11-01), DeVaughn
patent: 5844686 (1998-12-01), Treptow et al.
patent: 6071231 (2000-06-01), Mendoza et al.
patent: 6117394 (2000-09-01), Smith
Becton Dickinson and Company
Ludlow Jan
Thomas Nanette S.
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