Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Patent
1986-12-01
1988-09-27
Schain, Howard E.
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
530399, 530391, 530413, 436501, C08L 8900, C12P 2100
Patent
active
047743212
ABSTRACT:
A novel 100 kDa protein from Drosophila melanogaster (dp100) that recognizes both mammalian epidermal growth factor (EGF), insulin and insulin-related growth factors, and crossreacts with antisera against the human EGF receptor. The binding spectrum and relative binding affinities of dp100 for growth factors and hormones, related and unrelated to EGF or insulin, demonstrate that dp100 binds to insulin-like and EGF-like factors with dissociation constants ranging from 10.sup.31 6 M to 10.sup.-9 M. Dp100 binds to human synthetic transforming growth factor-alpha (TGF-alpha) and insulin-like growth factor-II with the highest affinity and, unlike the mammalian EGF receptor, has the unique ability to differentiate between EGF and TGF-alpha with a difference in affinity of three orders of magnitude. Further, dp100 is able to differentiate on the basis of binding affinity between native TGF-alpha, TGF-alpha that has been synthesized chemically and TGF-alpha that has been produced in E. coli using recombinant DNA technology. The specific competition of the EGF-like and insulin-like growth factors for dp100 provides a means for identifying or assaying for the presence of, and structural and functional modifications of, these growth factors and hormones, as well as a means for purification or removal of these substances.
REFERENCES:
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Thompson et al, Proc. Natl. Acad. Sci. USA, 82, 8443, Dec. (1985).
Livneh et al, Cell, 40(3), 599-607, (1985).
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Garcia J. Victor
Rosner Marsha R.
Stoppelli M. Patrizia
Thompson Karol L.
Kushan Jeff P.
Massachusetts Institute of Technology
Pabst Patrea L.
Schain Howard E.
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