Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – Via agrobacterium
Reexamination Certificate
2007-11-20
2007-11-20
Bui, Phuong (Department: 1638)
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
Via agrobacterium
C435S469000
Reexamination Certificate
active
10291504
ABSTRACT:
Development of an efficient and cost-effective doubled haploid production system and genetic transformation system are the prerequisite to initiate haploid breeding and genetic modification in flax respectively. Pre-culturing anthers on a high osmotic, high auxin and high mineral salt concentration for a period of time before transfer to a low osmotic, low auxin and low salt concentration significantly increased the overall efficiency of regeneration or anther efficiency than directly culturing anthers on a low osmotic, low auxin and low salt concentration medium. This culture procedure also dramatically reduced the frequency of shoot regeneration from somatic cells in anther culture. Using this procedure, a highly efficient anther culture-derived callus based transformation system was developed. The transformation efficiency of anther culture-derived callus based transformation system was four times higher than the best reported transformation efficiency using hypocotyls as the ex-plants inAgrobacterium tumefaciensbased transformation system or particle bombardment based transformation system. The frequency of escape in anther culture-derived callus based transformation system was one third of that in hypocotyl-based transformation system usingA. tumefaciensor one half using particle bombardment. This very high efficient transformation system will prove to be very valuable in basic research for gene discovery and practical applications in genetic engineering for improved traits.
REFERENCES:
patent: 5272072 (1993-12-01), Kaneko et al.
patent: 5750871 (1998-05-01), Moloney et al.
patent: 5994624 (1999-11-01), Trolinder et al.
patent: 6140553 (2000-10-01), D'Halluin
Baillie et al 1992 Plant Cell Reports 11:234-237.
Zhan et al 1988 Plant Molecular Biology 11:551-559.
Bretagne-Sagnard et al 1996 Transgenic Research 5:131-137.
Chen et al 1998 Plant Breeding 117:463-467.
Ferrie, A.M.R., “Evaluation ofBrassica rapaL. Genotypes for microspore culture response and identification of a highly embryogenic line.”,Plant cell reports; (1995), vol. 14, p. 580-584.
Baillie, A.M.R., “In vitro culture of isolated microspores and regeneration of plants inBrassica campestris.”,Plant cell reports; (1992), vol. 11, p. 234-237.
Chen, Y., “High frequency of plant regeneration from anther culter in flax,Linum usitatissimumL.”Plant breeding; vol. 117, (1998), p. 463-467.
Dunwell, J.M., “Role of sucrose in microspore embryo production inBrassica napusssp. Oleifera.”Journal of experimental botany; vol. No. 170, Sep. (1985), p. 1478-1491.
Guo, Y.D., “Isolated microspore culture and plant regeneration in rye (Secale cerealeL.).”Plant cell reports; (2000), vol. 19, p. 875-880.
Kuchuk, N., “Plant regeneration from leaf protoplasts of evening primrose (Oenothera hookeri).”Plant cell reports; (1998) vol. 17 p. 601-604.
Chen Yurong
Dribnenki Joseph Clifford Paul
Agrecore United
Battison Adrian D
Bui Phuong
Dupuis Ryan W.
Page Brent T
LandOfFree
Doubled haploid production and genetic transformation does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Doubled haploid production and genetic transformation, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Doubled haploid production and genetic transformation will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3820306