Double transposition methods for manipulating nucleic acids

Details Double transposition methods for manipulating nucleic acids Double transposition methods for manipulating nucleic acids

2008-03-04

2008-03-04

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

C435S476000, C435S488000, C536S023100

Reexamination Certificate

active

11369497

ABSTRACT:
Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each pair of transposase-interacting sequences interacts with a distinct transposase enzyme.

REFERENCES:
patent: 5925545 (1999-07-01), Reznikoff
patent: 5948622 (1999-09-01), Reznikoff
patent: 5965443 (1999-10-01), Reznikoff
patent: 6159736 (2000-12-01), Reznikoff
patent: 6294385 (2001-09-01), Reznikoff
patent: WO 98/10077 (1998-03-01), None
patent: WO 00/17343 (2000-03-01), None
patent: WO 01/09363 (2001-02-01), None
Bhasin A, et al., “Hairpin formation in Tn5 transposition,” J. Biol. Chem. 274:37021-37029 (1999).
Davies D, et al., “Three-dimensional structure of the Tn5 synaptic complex transposition intermediate,” Science 289:77-85 (2000).
Goryshin I, et al., “Tn5/IS50 target recognition,” Proc. Natl. Acad. Sci. USA 95:10716-10721 (1998).
Goryshin I & Reznikoff W, “Tn5 in vitro transposition,” J. Biol. Chem. 273:7367-7374 (1998).
Hoffman L, et al., “Transposome insertional mutagenesis and direct sequencing of microbial genomes,” Genetica 108:19-24 (2000).
Magarshak I, “Double transpositions and RNA secondary structures,” Biofizika. 38:886-887 (1993).
Naumann T & Reznikoff W, “Trans catalysis in Tn5 transposition,” Proc. Natl. Acad. Sci. USA 97:8944-8949 (2000).
Ostermeier M, et al., “A combinatorial approach to hybrid enzymes independent of DNA homology,” Nat. Biotechnol. 17:1205-1209 (1999).
Pollack A, “Selling evolution in ways darwin never imagined; if you can build a better gene, investors may come,” New York Times, Oct. 28, 2000, Health Section.
Sakai J, et al., “Identification and characterization of a pre-cleavage synaptic complex that is an early intermediate in Tn10 transposition,” EMBO J. 14:4374-4383 (1995).
Sieber V, et al., “Libraries of hybrid proteins from distantly related sequences,” Nat. Biotechnol. 19:456-460 (2001).
Zhou M & Reznikoff W, “Tn5 transposase mutants that alter DNA binding specificity,” J. Mol. Biol. 271:362-373 (1997).
Zhou M , et al., “Molecular genetic analysis of transposase-end DNA sequence recognition: cooperativity of three adjacent base-pairs in specific interaction with a muTn5 transposase,” J. Mol. Biol. 276:913-925 (1998).

Affiliated with

Also associated with

Rating

Double transposition methods for manipulating nucleic acids does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Double transposition methods for manipulating nucleic acids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Double transposition methods for manipulating nucleic acids will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3919221