Double transposition methods for manipulating nucleic acids

Details Double transposition methods for manipulating nucleic acids Double transposition methods for manipulating nucleic acids

2006-06-27

2006-06-27

Vogel, Nancy (Department: 1636)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S024000

Reexamination Certificate

active

07067644

ABSTRACT:
Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each pair of transposase-interacting sequences interacts with a distinct transposase enzyme.

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