Double transposition methods for manipulating nucleic acids

Details Double transposition methods for manipulating nucleic acids Double transposition methods for manipulating nucleic acids

2008-03-04

2008-03-04

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Process of mutation, cell fusion, or genetic modification

Introduction of a polynucleotide molecule into or...

C435S476000, C435S488000, C536S023100

Reexamination Certificate

active

07338801

ABSTRACT:
Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each pair of transposase-interacting sequences interacts with a distinct transposase enzyme.

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