Double targeted gene replacement in unicellular diploid organism

Details Double targeted gene replacement in unicellular diploid organism Double targeted gene replacement in unicellular diploid organism

1996-12-09

1999-09-21

Degen, Nancy

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

43525411, 4352583, C12N 1563, C12N 110, C12N 114

Patent

active

059553332

ABSTRACT:
Homozygous gene replacement can be created in unicellular diploid organisms by individually targeting each allele of a gene with genetic constructs containing two different and independent selectable markers. Selection for both markers indicates replacement of both alleles of the gene, or portion thereof. The method can be used to study gene function in these organisms and to create mutant organisms such as attenuated strains of parasitic protozoans for use in live vaccines.

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