Double-stranded vector and process using it

Details Double-stranded vector and process using it Double-stranded vector and process using it

1986-04-07

1989-09-05

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 4351723, 435320, 935 6, 935 77, C12Q 168, C12N 1500

Patent

active

048638480

ABSTRACT:
The invention concerns a double-stranded recombinant DNA cloning vector comprising one single insertion site for foreign DNA and one or two labelling sites adjacent or approximate to said insertion site. In the instance of two labelling sites, said sites flank the insertion site. The labelling site(s) can be individually labelled in a fashion that results in the labelling of the 3' end of either strand. The use of the invention in a process affords the ability to individually label only one strand at the 3' end in a simplified method for base sequencing analysis.

REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
Eckert, Gene 51:247-254, 1987.
Covarrubias, et al. Gene 13:25-35, 1981.
Davis et al., 1980, A Manuel for Genetic Engineering Advanced Bacterial Genetics, Cold Spring Harbor Laboratory, pp. 168-172, 226-229, 250.

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