Double recoverable block of function

Details Double recoverable block of function Double recoverable block of function

2004-07-15

2009-02-24

Fox, David T (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of using a plant or plant part in a breeding process...

Method of breeding using gametophyte control

C800S287000, C800S288000, C435S199000, C435S320100

Reexamination Certificate

active

07495148

ABSTRACT:
An improved double Recoverable Block of Function (RBF) system for transgene containment with enhanced reliability is disclosed. The system includes two blocking constructs (BCs) at the opposite sides of a transgene of interest (TGI). The system comprises means for recovery of the function blocked by expression of the blocking genes of the BCs. The invention is also related to a kit comprising a cloning vector having the BCs and TGI or alternatively a place for TGI to be inserted in. Segregating (two-insert) and inducible (one-insert) types of double RBF are disclosed. The two BCs are either similar or different in their DNA and RNA structures. The two BCs may encode same protein but promoters driving the blocking genes are different and/or the coding sequence of the blocking genes is modified by codon preference change. A system comprising two BCs having different blocking genes is also disclosed. The system may consist of one or two Recovering constructs (RC), but also a system recovering without an RC is disclosed. Whether the system includes an RC or not, the recovery is induced by an external intervention that is controlled by human being.

REFERENCES:
patent: 6849776 (2005-02-01), Kuvshinov et al.
patent: WO97/44465 (1997-11-01), None
patent: WO02/06498 (2002-01-01), None
patent: WO03/076633 (2003-09-01), None
Kuvshinov et al. Environmental Biosafety Research 4(2): 103-112 (Apr.-Jun. 2005).
Kuvshinov et al. Plant Science 167(1): 173-182 (Jul. 2004).
Kriete et al. The Plant Journal 9(6): 809-816 (1996).
Williams, M.E. TIBTECH 13: 344-349 (Sep. 1995).
U.S. Appl. No. 09/371,307, filed May 2, 2002, Brown et al.
Kuvshinov, Viktor; Koivu, Kimmo, Kanerva, Anne and Pehu, Eija Molecular control of transgene escape from genetically modified palnts. Plant Science 160 (2001) 517-522.
Kuvshinov , V, et al. 2005. Double recoverable block of function- . . . Environ, Biosafety res. E: 103-112.
Schernthaner, Control of seed germination in transgenic plants . . . PNAS vol. 100, No. 11; May 27, 2003, pp. 6855-6859.
Gressel, J. Tandenm constructs: preventing the rise of superweeds. TIBTECH 1999 (vol. 17) pp. 361-366.
Daniel, H. (2002) Molecular Strategies for gene containment in transgenic crops. Nature Biotechnology vol. 20 p. 581-586.

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