Double receptor polynucleotide assay method

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 912, 435 911, 435810, 435 71, 435 79, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 536 268, 530350, C12P 1934, C12Q 168, G01N 3353, C07H 2104

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060635657

ABSTRACT:
A method for the detection of a polynucleotide target sequence is described. The method involves the formation of a covalent or non-covalent bonded pair of nucleotide sequences formed in response to a target polynucleotide sequence, adding nucleotide sequence specific binding proteins each capable of binding one member of the pair of nucleotide sequences, and detecting the specific binding proteins completed to the pair of nucleotide sequences.

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patent: 4775619 (1988-10-01), Urdea
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patent: 4868104 (1989-09-01), Kurn et al.
patent: 5185243 (1993-02-01), Ullman et al.
Kemp, et al., "Colorimetric detection of specific DNA segments amplified by polymerase chain reactions," Proc. Natl. Acad. Science USA, vol. 86, pp. 2423-2427, Apr. 1989.

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