Drug – bio-affecting and body treating compositions – Identification or warning feature – Taste or smell or chemical irritation to the eye – nose – or...
Patent
1973-10-02
1976-12-21
Wolk, Morris O.
Drug, bio-affecting and body treating compositions
Identification or warning feature
Taste or smell or chemical irritation to the eye, nose, or...
23230B, 1951035R, 260112B, 25230116, G01N 2100, G01N 3316
Patent
active
039989437
ABSTRACT:
A sensitive method for determining qualitatively and quantitatively the presence of a wide variety of physiologically active organic compounds (ligand) is provided. The method employs a reagent which involves bonding a compound having structural similarity to the compound to be determined (ligand analog) to a fluorescing compound. The unknown compound is referred to as a ligand, the conjugate of the structurally similar compound and fluorescer is referred to as ligand analog-fluorescer, and compounds which recognize a specific structure and bind to such structure are referred to as receptors and are normally antibodies.
The fluorescer which is chosen will have either a change in quantum yield or a change in its emisson and/or absorption spectra or all of them, when bound to antibody, as compared to being unbound. For the purposes of the assay, all that is required is that there be a change in the emisson intensity at some wavelength or band of wavelengths.
The rate at which fluorescer antibody binds to the fluorescer portion of the ligand anaog-fluorescer or the amount of fluorescer antibody bound to the fluorescer portion of the ligand analog-fluorescer at equilibrium will be related to the amount of ligand antibody bound to the ligand analog portion of the ligand analog-fluorescer. Therefore, by combining antibodies to both ligand and fluorescer, with ligand analog-fluorescer and an unknown, one can determine the amount of ligand present in the unknown by relating the emission intensity at a particular wavelength or band of wavelengths to standards.
REFERENCES:
patent: 3656906 (1972-04-01), Bullock
patent: 3709868 (1973-01-01), Spector
patent: 3766162 (1973-10-01), Spector
patent: 3775536 (1973-11-01), Spector
patent: 3789116 (1974-01-01), Kay
patent: 3809782 (1974-05-01), Spector
patent: 3817706 (1974-06-01), Smith
patent: 3901654 (1975-08-01), Gross
patent: 3935074 (1976-01-01), Rubenstein
Chemical Abstracts, 73:64436m (1970).
Chemical Abstracts, 73:48541m (1970).
Chemical Abstracts, 79:101164t (1973).
Marantz Sidney
Syva Company
Wolk Morris O.
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